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SNP Molecular Markers Related to Maize Row Kernel Number and Its Application

A molecular marker, row number technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc. process, the effect of improving accuracy

Active Publication Date: 2022-06-28
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the QTL mapping region generally spans a large span, usually several centimorgans (cM), and the mapping region includes a large number of candidate genes, and the fine mapping of genes is difficult
The candidate gene method is based on the existing life science background knowledge, directly selects the genes that may affect the trait from the known or potential gene pool, but its disadvantage is that it cannot identify new genes

Method used

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  • SNP Molecular Markers Related to Maize Row Kernel Number and Its Application
  • SNP Molecular Markers Related to Maize Row Kernel Number and Its Application
  • SNP Molecular Markers Related to Maize Row Kernel Number and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]1.1 Construction of GWAS groups

[0027] The maize genome-wide association analysis population includes 431 inbred lines with different kinship. All inbred lines are provided by the School of Plant Science, Jilin University, and are planted in the Teaching and Research Experimental Base of the School of Plant Science, Jilin University (Lvyuan District, Changchun City, Jilin Province). The arrangement of the plots followed a random block design, the block was repeated 3 times, the single-row plot, the row length was 3 m, the row spacing was 65 cm, and the plant spacing was 20 cm. The field management measures were the same as those in the field.

[0028] 1.2 Collection of samples and investigation of phenotypic data

[0029] At the 5-6 leaf stage of the maize seedlings in the plot, 3 seedlings were collected, cleaned and stored in a -20°C refrigerator. Five representative ears were harvested from each plot, and the number of kernels per row (KPR) was examined after the ...

Embodiment 2

[0041] 2.1 Construction of sequencing library

[0042] (1) 200ng of genomic DNA was digested with the restriction enzyme EcoRI, and after the digestion was complete, it was purified and recovered by magnetic beads.

[0043] (2) The purified DNA after enzyme digestion was connected to Barcode adapters PI with T4 DNA Ligase, the ligation product was purified and recovered by magnetic beads, and the qualified P1 primer label was recorded.

[0044] (3) All samples were mixed in equal proportions into a total DNA mix, and the DNA was fragmented using covaris220. The fragment length of the fragmented DNA was about 200-500 bp.

[0045] (4) After End Repair & dA-tailing, connect to Adaptor P2, and purify and recover the ligated product with magnetic beads.

[0046] (5) Use the KAPA 2G Robust PCR Kit to amplify and enrich the linker ligation products, use magnetic beads to purify and sort the PCR reaction products, and use 2% agarose gel electrophoresis to detect the quality of the co...

Embodiment 3

[0063] Intergroup comparison of different genotypes detected at significant loci

[0064] 3.1 Zm00001d018060 gene Chr5:213511022 locus comparison between groups

[0065] This locus includes three genotypes, namely C / C, T / T, and C / T. The box plots of the row grain number distribution of samples of different genotypes are shown in Figure 5 . The median of C / C group was 25.77 grains, the lower quartile Q1 was 22.83 grains, and the upper quartile Q3 was 29.77 grains; the T / T group had a median of 28.66 grains, and the lower quartile Q1 was 24.77 grains grains, the upper quartile Q3 was 31.00 grains; the median of C / T group was 28.55 grains, the lower quartile Q1 was 25.00 grains, and the upper quartile Q3 was 32.05 grains.

[0066] The t-test results of the phenotypic mean difference between different genotype groups are shown in Table 2. It can be seen from Table 2 that the difference between the C / C and T / T groups is extremely significant (P=0.0001), and the mean difference b...

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Abstract

The SNP molecular marker related to corn row kernel number and its application belong to the technical field of crop genetic breeding molecular marker application technology, and the invention specifically relates to the SNP molecular marker and application related to two row kernel number traits on the No. 5 chromosome of corn. One of the SNP molecular markers is located in the intron region of gene Zm00001d018060, and the other SNP molecular marker is located in the coding region of gene Zm00001d013521. Both SNP molecular markers are obtained from genome-wide association analysis of maize inbred populations. The acid sequences are respectively shown in: SEQIDN01 and SEQIDN02. The SNP molecular marker of the present invention can be used for auxiliary selection of the trait of grain number in a row in the process of corn breeding, which can improve the accuracy of selection and speed up the process of cultivating new varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular marker application of crop genetics and breeding, and particularly relates to a SNP molecular marker related to the number of corn rows and its application. Background technique [0002] Corn is an important food and feed crop, which is of great significance to ensuring national food security. The yield of corn is determined by the number of ears per unit area, the number of rows per ear, the number of grains per row, and the weight of grains per ear, among which the number of rows of grains contributes more than 30% to the yield, which is the main component of corn yield. . For example, Xianyu has 38-40 grains in 335 rows, and Zhengdan has 42-46 grains in 958 rows. Therefore, excellent corn varieties have more grains in rows as a guarantee for high grain yield. Grain number is a typical quantitative trait controlled by polygenes, and its heritability in both broad and narrow senses is relativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11G16B20/50
CPCC12Q1/6895G16B20/50C12Q2600/156C12Q2600/13
Inventor 胡军潘洪玉甘会云丁韦辰王世壮王寒王鹏葳陈逸飞金天于汇琳张鑫生唐心龙王成
Owner JILIN UNIV
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