Apios americana Medikus flower polysaccharide and preparation method thereof

A technology of bean taro flower and bean taro, applied in a method and the field of polysaccharide obtained, can solve problems such as complex structure, achieve high purity, great application value, and low energy consumption

Inactive Publication Date: 2017-11-17
ZHEJIANG UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sugar chain combination of plant polysaccharides is mainly β-1,3 or β-1,6 bonds, and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Apios americana Medikus flower polysaccharide and preparation method thereof
  • Apios americana Medikus flower polysaccharide and preparation method thereof
  • Apios americana Medikus flower polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, a kind of preparation method of taro flower polysaccharide, carries out following steps successively:

[0063] 1), preparation of taro flower polysaccharide crude product:

[0064] According to the solid-liquid ratio of 1g:5ml, weigh 100g of taro flower and add 500mL of distilled water, extract in 90°C water bath for 80min (enzyme inactivation is also achieved during extraction), then beat, and then centrifuge (4000r / min for 15min), and Suction filter the supernatant obtained by centrifugation and collect the filtrate;

[0065] Use the filter residue obtained by centrifugation to replace the taro flower, repeat the above process of adding distilled water, then extracting (90°C water bath for 2 hours), beating, and centrifuging twice, and combine the filtrate obtained by centrifuging and suctioning for three times to concentrate to the original volume. 1 / 5 of the concentrated solution.

[0066] Under the condition of stirring, add ethanol (pure ethanol) t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Relative molecular massaaaaaaaaaa
Weight average relative molecular massaaaaaaaaaa
Login to view more

Abstract

The invention discloses Apios americana Medikus flower polysaccharide and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a crude product of Apios americana Medikus flower polysaccharide, namely mixing Apios americana Medikus flowers with distilled water, sequentially carrying out extraction, pulping, centrifugation and concentration, so as to obtain concentrated liquid, adding ethanol into the obtained concentrated liquid until the final concentration of the ethanol is 80+/-5%, standing at 4+/-1 DEG C for 24+/-2h, and collecting precipitates, so as to obtain the crude product of the Apios americana Medikus flower polysaccharide; and (2) purifying the Apios americana Medikus flower polysaccharide, namely carrying out activated carbon decoloration and Sevage-method deproteinization on the Apios americana Medikus flower polysaccharide. The prepared Apios americana Medikus flower polysaccharide contains a beta-glucosidic bond, a pyranose ring, glucose, fructose and xylose, and the molar ratio of glucose to fructose and to xylose is 1.79 to 1 to 0.03.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for extracting and purifying polysaccharides from bean taro flowers and the obtained polysaccharides. Background technique [0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitami...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 郑晓冬楚强陈鹏华
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap