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Porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as preparation method and application thereof

A technology for swine influenza virus and respiratory syndrome, applied in the direction of virus/bacteriophage, biochemical equipment and methods, vaccines, etc., can solve the problem of difficulty in maintaining the immunogenicity and safety of inactivated vaccines, the reduction of active components of vaccines, and the reduction of efficacy, etc. problems, to achieve the effects of enhancing local and systemic immune responses, enhancing presentation, and prolonging the immune period of vaccines

Active Publication Date: 2017-11-24
CHINA AGRI VET BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Highly purified vaccine antigens, because the vaccine formula contains trace amounts of residual impurity proteins and nucleic acids, which eliminates potential allergic reactions, but on the other hand, it will cause degradation, adsorption or aggregation of purified inactivated viruses, thereby reducing the active components of the vaccine and reducing the effect , so excipients need to contain stable adjuvants and antigen stabilizers. At present, it is difficult to maintain the immunogenicity and safety of inactivated vaccines with single aluminum-stabilized adjuvants and two-way oil-stabilized adjuvants for vaccines. Therefore, it is necessary to develop A stable adjuvant for long-term vaccines that can induce specific humoral and cellular immunity

Method used

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  • Porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as preparation method and application thereof
  • Porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as preparation method and application thereof
  • Porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 Preparation of porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virus particles and vaccine

[0086] 1. Virus strains, cells, culture medium

[0087] The vaccine and the PRRSV antibody strain prepared by the present invention are the 25th generation virus strain HP-PRRSV-JXM-F25 of the highly pathogenic porcine reproductive and respiratory syndrome virus HP-PRRSV-JXM-F5 strain passed on Marc-145 cells. Highly pathogenic porcine reproductive and respiratory syndrome virus HP-PRRSV-JXM-F5 strain, which is an adapted strain of HP-PRRSV-JXM uploaded to the fifth generation in MARC-145 cells, is classified as highly pathogenic porcine reproductive and respiratory syndrome virus. Respiratory Syndrome Virus, its microbial preservation number is: CGMCCNO.9453, preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, the preservation date is July 8, 2014, and the preservation ad...

Embodiment 2

[0151] Example 2 Stable Adjuvant Complex, Porcine Reproductive and Respiratory Syndrome Whole Virus-Swine Influenza Reconstituted Virosome Vaccine Preparation and Quality Control Method

[0152] 1. Preparation and determination of BCG lysate

[0153] Bacillus Calmette-Guerin (BCG) D2BP302S11 was provided by the China National Institute for the Control of Pharmaceutical and Biological Products, and seed batches were established according to the management regulations for strains used in the production and testing of biological products. It should not exceed 12 generations from the batch of working seeds to the collection of bacteria. On the Sutong medium, BCG should float on the surface, and it is a wrinkled and yellow bacterium. The results of toxicity test and non-toxic BCG test were negative. Freeze-dried strains are stored at 2-8°C, and liquid strains are stored below -70°C.

[0154] Bacillus Calmette-Guerin D2BP302S11 was cultured statically at 37°C for 2-3 weeks with mo...

Embodiment 3

[0184] Example 3 Stability and Validity of Porcine Reproductive and Respiratory Syndrome Complete Virus-Swine Influenza Virus Reconstituted Virosome Vaccine

[0185] 3.1 Stability of vaccine semi-finished products in liquid frozen state

[0186] The stock solution of porcine reproductive and respiratory syndrome complete virus-swine influenza virus reconstituted virion vaccine was prepared according to the method in Example 1, and the preparation method of the stable adjuvant complex and vaccine antigen was prepared according to the method in Example 2.

[0187] The vaccine semi-finished products were stored in polypropylene bottles at -35°C and -70°C, and the inactivated and purified porcine reproductive and respiratory syndrome intact virus-swine influenza virus reconstituted virion particles were tested every 3 months. The results are shown in Table 1 below.

[0188] Table 1 Stability of vaccine semi-finished products stored in frozen state

[0189]

[0190]

[0191]...

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Abstract

The invention discloses a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as a preparation method and an application thereof. The vaccine consists of porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion antigen and a stable adjuvant compound; a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion is formed through direct adsorption or covalent binding of porcine reproductive and respiratory syndrome virus to the surface of a swine influenza virus reconstructed virion, wherein the swine influenza virus reconstructed virion is a small vesicle containing a phospholipid bilayer; and haemagglutinin and neuraminidase are bound to the surface of the phospholipid bilayer. The vaccine prepared by the invention has the characteristics of being safe, efficient, convenient to use, long in efficacy time and the like. Purposes of reducing and / or removing respiratory syndrome virus and / or swine influenza virus can be achieved through intranasal immunization.

Description

technical field [0001] The invention relates to a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and its preparation method and application. The invention belongs to the technical field of biological medicine or veterinary biological products. Background technique [0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is a chronic disease of pigs. It is prevalent in countries that raise pigs around the world, causing economic losses of about 40 billion a year. It is one of the most important infectious diseases in pig farming and meat industry . [0003] Since the discovery of PRRSV virus, inactivated and attenuated modified live vaccines (Modified live, PRRS-MLV) have been developed at home and abroad for the control of PRRSV. Nevertheless, PRRS-MLV has the risk of recombination and mutation for susceptible pigs, and the live vaccine strains may also have the poss...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/145A61K39/12A61K39/39A61P31/16A61P31/14
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/5258A61K2039/543A61K2039/55583A61K2039/55594A61K2039/57A61K2039/575A61K2039/70C12N2710/10034C12N2760/16034
Inventor 吕宏亮董金杰张杰田波邓瑞雪王会保刘萍刘西兰景志忠高世杰王超英张云德张永光殷宏
Owner CHINA AGRI VET BIO SCI & TECH
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