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A method for preparing ginsenoside rz1 based on biogenic degradation pathway

A technology of ginsenoside and RZ1, which is applied in the field of preparation of ginsenoside RZ1 based on biogenic degradation pathways, can solve the problems of limiting the research on the pharmacological effects of ginsenoside RZ, low content, and difficult separation of standard products

Inactive Publication Date: 2018-09-18
瑞莱茵(北京)生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, since ginsenoside RZ 1 Relatively no RK 1 , RG 5 Stable, so the content is low, the separation of standard products is difficult, and it also limits ginsenoside RZ 1 Pharmacological studies of

Method used

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  • A method for preparing ginsenoside rz1 based on biogenic degradation pathway
  • A method for preparing ginsenoside rz1 based on biogenic degradation pathway
  • A method for preparing ginsenoside rz1 based on biogenic degradation pathway

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: Ginsenoside Rb 1 Preparation of Ginsenoside RZ by Thermal Degradation 1

[0027] 1. Preparation of raw material solution

[0028] Ginsenoside Rb 1 Standard product (Shaanxi Huikangyuan Biomedical Technology Co., Ltd., purity > 98%) and D-alanine (Bailingwei Technology Co., Ltd., purity 99%) are dissolved in 30% ethanol aqueous solution to make ginsenoside Rb 1 The mass concentration is the solution of 5mg / mL, and the molar concentration of D-alanine is Rb 1 25% of the molar concentration.

[0029] 2. Thermal degradation method

[0030] The obtained raw material solution was transferred to a 100mL polytetrafluoroethylene-lined stainless steel reactor, and placed in a constant temperature oven for hydrothermal reaction at a reaction temperature of 170°C and a reaction time of 5 hours. After the reaction was completed, the reactor was taken out and cooled to room temperature.

[0031] Determination of Ginsenoside RZ in Thermal Degradation Products ...

Embodiment 2

[0039] Embodiment 2: Ginsenoside Rb 2 Preparation of Ginsenoside RZ by Thermal Degradation 1

[0040] 1. Preparation of raw material solution

[0041] Ginsenoside Rb 2 Standard substance (Shanghai Rongbai Biotechnology Co., Ltd., purity > 98%) and D-alanine (Bailingwei Technology Co., Ltd., purity 99%) are prepared by dissolving ginsenoside Rb with a volume percentage concentration of 30% ethanol solution 2 The mass concentration is the solution of 5mg / mL, and the molar concentration of D-alanine is Rb 2 25% of the molar concentration.

[0042] 2. Thermal degradation method

[0043] The obtained raw material solution was transferred to a 100mL polytetrafluoroethylene-lined stainless steel reactor, and placed in a constant temperature oven for hydrothermal reaction at a reaction temperature of 170°C and a reaction time of 5 hours. After the reaction was completed, the reactor was taken out and cooled to room temperature.

[0044] Determination of Ginsenoside RZ in The...

Embodiment 3

[0051] Embodiment 3: Ginsenoside Rb 3 Preparation of Ginsenoside RZ by Thermal Degradation 1

[0052] 1. Preparation of raw material solution

[0053] Ginsenoside Rb 3 Standard substance (Shanghai Valan Biotechnology Co., Ltd., purity > 98%) and D-alanine (Bailingwei Technology Co., Ltd., purity 99%) are prepared by dissolving ginsenoside Rb with a volume percentage concentration of 30% ethanol solution 3 The mass concentration is the solution of 5mg / mL, and the molar concentration of D-alanine is Rb 3 25% of the molar concentration.

[0054] 2. Thermal degradation method

[0055] The obtained raw material solution was transferred to a 100mL polytetrafluoroethylene-lined stainless steel reactor, and placed in a constant temperature oven for hydrothermal reaction at a reaction temperature of 170°C and a reaction time of 5 hours. After the reaction was completed, the reactor was taken out and cooled to room temperature.

[0056] Determination of Ginsenoside RZ in Therm...

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Abstract

The invention relates to a method for preparing ginsenoside RZ1 based on source degradation pathways. The method comprises the following steps: firstly adopting protopanoxadiol saponins as materials to carry out thermal degradation, and then separating and purifying thermal degradation products to obtain the ginsenoside RZ1. The method comprises the detailed steps of dissolving the protopanoxadiol saponin by using water, adding a cosolvent or not, adding an isomer selecting agent, preparing into material solution, and then carrying out thermal degradation, wherein the isomer selecting agent is D-alanine. The method provided by the invention has the advantages that the protopanoxadiol saponins (such as ginsenoside Rb1, Rb2, Rb3, Rc, Rd and Rg3) can be selectively thermally-degraded into the ginsenoside RZ1, and the conversion rate (the conversion rate is equal to a product of actual conversion mole number / maximum theoretical conversion mole number and 100%) of the ginsenoside RZ1 is about 90%, so that the method can be used for specifically preparing the ginsenoside RZ1.

Description

technical field [0001] The present invention relates to the preparation of ginsenosides, in particular to a preparation of ginsenoside RZ based on biogenic degradation pathways 1 Methods. Background technique [0002] The applicant found that three ginsenosides can promote osteogenic differentiation of bone marrow mesenchymal stem cells, namely ginsenoside RK 1 , RG 5 and RZ 1 . Ginsenoside RK 1 , RG 5 It was first discovered by Korean scholar II Ho Park et al. (Reference: Three New Dammarane Glycosides from Heat Processed Ginseng, Arch Pharm Res Vol 25, No 4,428-432,2002), RZ 1 It was first discovered by Korean scholar Sang Myung LEE (reference: Ginsenosides from Heat Processed Ginseng, ChemPharmBull, 2009). These three ginsenosides were all isolated from heat-processed ginseng, and the three are isomers. Ginsenoside RG 3 Dehydrated. [0003] Ginsenoside RK 1 , RG 5 and RZ 1 The chemical structural formula and its biodegradation pathway are as follows: [0004]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00C07B53/00
CPCC07B53/00C07B2200/07C07J17/005
Inventor 孙玉鹤杨勇彭雨泽
Owner 瑞莱茵(北京)生物科技有限责任公司
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