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Method for producing edible fungus rejuvenating-type strain

A technology of edible mushrooms and strains, which is applied in the direction of fungi, etc., can solve the problems of strain degradation, etc., and achieve the effects of obvious mushrooming tide, high growth synchronization, and increased mycelium per unit volume

Active Publication Date: 2017-11-24
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the strain degradation problem existing in the current edible fungus production, adopt tissue separation combined with single hyphae purification method, carry out the production of rejuvenating strains suitable for the actual industrial development needs of edible fungi, and provide a better solution to the strain degenerate method

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  • Method for producing edible fungus rejuvenating-type strain
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  • Method for producing edible fungus rejuvenating-type strain

Examples

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Embodiment 1

[0027] In 2015, at the edible fungi production base of Shanxi Agricultural University, the method of the present invention was used to obtain the rejuvenating mushroom 808 strain. The production method of rejuvenating type shiitake mushroom strain, concrete steps are as follows:

[0028] (1) Selection of seed mushrooms The seed mushrooms used for the production of rejuvenating shiitake mushroom strains require ideal mushroom shape, vigorous growth, and fruiting bodies free of insects and diseases. Usually, young mushrooms with pellicles connected to the stipe are selected when the organ differentiation has not yet completed (see figure 1 ).

[0029] (2) Tissue separation Rinse the selected seed mushrooms with sterile water, sterilize the surface with 75% alcohol solution, and cut longitudinally from the middle of the stipe or cap with a surgical blade burned by flame under aseptic conditions , tear it apart, pick a tissue block with a diameter of 3-5 mm at the junction of the...

Embodiment 2

[0039] In 2016, at the edible fungus production base of Shanxi Agricultural University, the method of the present invention was used to obtain the No. 1 rejuvenating Jinlingzhi strain. Most of the steps in the production method of the rejuvenating strain of Jinlingzhi No. 1 are the same as shiitake mushrooms, but in the selection of seed mushrooms, it is necessary to choose young mushrooms with milky white edges on the top of the fruit body and undeveloped caps (such as Figure 11 ), the ganoderma lucidum fruiting medium used was: 63% broad-leaved wood chips, 15% cottonseed hulls, 10% bran, 5% rice bran, 1% gypsum powder, 1% superphosphate, and the ratio of material to water was 1:1.1.

[0040] All the other operating steps are the same as in Example 1.

[0041] The results showed that the rejuvenated Jinlingzhi No. 1 strain obtained by this method had high growth synchronization, the growth rate increased by 10.8% compared with that before rejuvenation, the mycelium per unit ...

Embodiment 3

[0043] In 2017, at the edible fungus production base of Shanxi Agricultural University, the method of the present invention was used to obtain the rejuvenating type Hericium 96 strain.

[0044] Hericium erinaceus is a type of edible fungus of the family Hericium erinaceae of the order Russulata, named for its physical shape resembling a hedgehog or Hericium erinaceus. The seed mushrooms used for the production of rejuvenating strains require fruiting bodies with ideal mushroom shape, vigorous growth, and no insects and diseases. Usually, young fruiting bodies with rounded, head-shaped, solitary, dense, sessile, short spines, and 5-8cm in diameter are selected. Mushrooms (see Figure 12) Hericium erinaceus mother species culture medium: use 200g of potatoes, 20g of glucose, 20g of agar, 2g of citric acid, and 1000mL of water to prepare a PDA sterile medium culture dish and a PDA slant medium, and the diameter of the culture dish is 90mm; The base is: 85% cottonseed hulls, 7% r...

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Abstract

The invention discloses a method for rejuvenating an edible fungus strain. The method comprises strain mushroom selection, tissue isolation, single mycelium separation, transferring and rejuvenating, culture material screening, fruiting verification and pilot scale test in production. Compared with the prior art, the method fully utilizes biological characteristics of the mycelium growth of the edible fungi and utilizes combination of typical single mycelium cell separation under an optical microscope and production culture medium screening. The rejuvenated fungus strain obtained by the method has high growth synchronism, has a growth rate increased by about 10-20% than that before rejuvenating and has the mycelial volume per unit volume increased by 15 to 25% and has obvious fruiting frequency.

Description

technical field [0001] The invention relates to the fields of edible fungus cultivation properties and strain production, in particular to a screening and production method of edible fungus rejuvenation strains. Background technique [0002] Edible fungi are known as "mountain treasures" with high nutritional and medicinal values. They contain 8 essential amino acids that the human body cannot synthesize by themselves, especially active ingredients such as fungal polysaccharides, glycopeptides, and triterpenes, which have anti-oxidation and anti-aging properties. , enhance immunity, inhibit tumor and anticancer effects. With the improvement of people's understanding of the nutritional and medicinal value of edible fungi, the consumption demand will increase. The total output of edible fungi in my country accounts for more than 70% of the world's total output, ranking fifth after grain, vegetables, fruits, and oil, and has become one of the leading industries of modern agric...

Claims

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Application Information

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IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 刘靖宇常明昌孟俊龙张敏徐丽婧王术荣耿雪冉
Owner SHANXI AGRI UNIV
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