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Application of CCBE1 protein to preparation of rectal cancer postoperative prognosis evaluation kit, and kit and method for rectal cancer prognosis evaluation

A CCBE1, 1.CCBE1 technology, applied in the field of rectal cancer prognosis assessment kits, can solve problems such as unclear role

Inactive Publication Date: 2017-11-24
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of CCBE1 in tumors is currently unclear

Method used

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  • Application of CCBE1 protein to preparation of rectal cancer postoperative prognosis evaluation kit, and kit and method for rectal cancer prognosis evaluation
  • Application of CCBE1 protein to preparation of rectal cancer postoperative prognosis evaluation kit, and kit and method for rectal cancer prognosis evaluation
  • Application of CCBE1 protein to preparation of rectal cancer postoperative prognosis evaluation kit, and kit and method for rectal cancer prognosis evaluation

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A kit for postoperative prognosis evaluation of rectal cancer, the kit includes: human CCBE1 monoclonal antibody or CCBE1 polyclonal antibody for detecting the relative expression of CCBE1 protein in rectal cancer tissue, immunohistochemical experiment reagent. Among them, immunohistochemical reagents include: xylene, ethanol, 3% H 2 o 2 , 3% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase, PBS and 0.01M EDTA repair solution.

[0043] The human CCBE1 polyclonal antibody of the present invention is obtained by immunizing rabbits with the CCBE1 protein whose sequence is shown in SEQ ID NO.1. It can be prepared by itself, or commercially available products can be used.

[0044] Immunohistochemical experiment reagent of the present invention comprises: Xylene, ethanol, 3%H 2 o 2 (aqueous solution), 3% BSA blocking solution (prepared in PBS), DAB chromogenic reagent, hematoxylin, horseradish peroxidase (for labeling secondary antibody...

Embodiment 2

[0046] The application of CCBE1 protein in the preparation of the postoperative prognosis evaluation kit of rectal cancer, the application is to use the kit of the present invention to evaluate the prognosis of rectal cancer postoperatively, the method is as follows:

[0047] (1) Take pathological specimens from patients with rectal cancer, and use human CCBE1 monoclonal antibody or human CCBE1 polyclonal antibody, as well as immunohistochemical reagents, for immunohistochemical staining;

[0048] Among them, the pathological specimens come from the biopsy tissue of rectal cancer patients or the pathological materials obtained during and after the operation. The immunohistochemical method uses the SP staining method, and the specific steps are as follows:

[0049] (a) Paraffin sections of rectal cancer tissues were prepared, and the oven was set at 60°C overnight.

[0050] (b) Dewaxing of slices. Soak in sequence: Xylene I: 10min; Xylene II: 10min; Xylene III: 10min.

[005...

Embodiment 3

[0069] A postoperative tumor sample of rectal cancer was taken for paraffin-embedded section, and detected by the above-mentioned immunohistochemical method. After calculation, the CCBE1 immunohistochemical score of the cancer tissue was 0.436. After postoperative follow-up, it was found that the patient developed rectal cancer recurrence and metastasis 11 months after the operation, and died 20 months after the operation.

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Abstract

The invention provides the application of the CCBE1 protein in the preparation of a kit for evaluating the prognosis of rectal cancer, the kit and the method for evaluating the prognosis of rectal cancer. The present invention detects the relative expression level of CCBE1 protein in rectal cancer tissue by immunohistochemical method, so as to judge the risk of rectal cancer recurrence and metastasis in rectal cancer patients. The beneficial effects of the present invention are mainly reflected in: the present invention provides the application of CCBE1 protein in the preparation of the postoperative prognosis assessment kit for rectal cancer, suggesting that CCBE1 protein can be used to prepare protein molecular markers for judging the prognosis of rectal cancer patients. Postoperative monitoring and sequential treatment also have important guiding significance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of CCBE1 protein in the preparation of a kit for evaluating the prognosis of rectal cancer, the kit and the method for evaluating the prognosis of rectal cancer. Background technique [0002] Rectal cancer refers to the cancer between the dentate line and the rectosigmoid junction, and is one of the common malignant tumors of the digestive tract. Rectal cancer is low in location and is easily diagnosed by digital rectal examination and sigmoidoscopy. However, because of its location deep into the pelvic cavity, the anatomical relationship is complicated, the operation is not easy to complete, the recurrence rate is high after operation, and the middle and lower rectal cancer is close to the anal canal sphincter, so it is difficult to preserve the anus and its function during operation. One of the most debated diseases. The median age of onset of rectal ca...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 林尧王清水
Owner FUJIAN NORMAL UNIV
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