Identification methods and uses of seed plant species

An identification method and technology for gymnosperms, applied in the field of biological detection, can solve problems such as being too large, not considering the genetic distance between species, etc., and achieve the effects of high application value, high accuracy, and low coverage requirements.
CN107400723BInactive Publication Date: 2019-12-06SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2019-12-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an identification method and application of a seed plant species, comprising the following steps: selecting a seed plant species, obtaining the ITS2 gene sequence of the seed plant species, constructing an evolutionary tree based on the ITS2 gene sequence, and selecting multiple pairs of sisters in the evolutionary tree Species, calculate the genetic distance between each pair of sister species, conduct a significant difference test on the genetic distances of multiple groups belonging to the same species, select the genetic distance with no significant difference to calculate the average value to obtain the molecular cut-off value; calculate the test species and the target The genetic distance between species is compared with the molecular cut-off value of the phylum to which the species to be tested is compared to determine the species category. The method of the present invention only needs to compare the degree of divergence between the ITS2 gene sequence of the sample to be tested or the suspected species and the target species, which is simpler and easier to implement, with high accuracy and strong objectivity, and does not require a large number of samples of other species of the same genus as For reference, it has high application value.
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Description

technical field

[0001] The invention relates to the technical field of biological detection, in particular to an identification method and application of a seed plant species. Background technique

[0002] At present, the main method of species identification is morphological classification. However, because of its poor accuracy, strong subjectivity and low efficiency, it can no longer meet the requirements of today's species classification and identification. With the improvement of sequencing level and the reduction of sequencing cost, the role of DNA sequence in species identification is becoming more and more prominent. In 2003, Canadian zoologist Paul Hebert first proposed the concept of DNA barcoding (DNA barcoding). It uses a standard, sufficiently variable, easy-to-amplify and relatively short DNA fragment to achieve rapid, accurate and automated species identification by establishing a biological DNA barcode database. DNA barcoding technology is currently the most...

Claims

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