Preparation of soluble squid ink melanin after fishy smell removal, and application of soluble squid ink melanin after fishy smell removal in foods

A technology of melanin and squid ink, which is applied in food ingredients as antioxidants, food science and other directions, can solve the problems of deodorization of squid ink, unsuitable products for processing and production, etc., and achieves high application potential, abundant raw materials, and simple preparation process. Effect

Active Publication Date: 2017-12-01
JIANGNAN UNIV
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some documents report that it utilizes ultrasonic extraction technology in lye to extract soluble squid ink melanin, but its purpose is to discuss the antioxidant properties of soluble squid ink melanin, a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of soluble squid ink melanin after fishy smell removal, and application of soluble squid ink melanin after fishy smell removal in foods
  • Preparation of soluble squid ink melanin after fishy smell removal, and application of soluble squid ink melanin after fishy smell removal in foods
  • Preparation of soluble squid ink melanin after fishy smell removal, and application of soluble squid ink melanin after fishy smell removal in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Thaw the squid ink sac at 0°C, soak it in the ice-water mixture overnight to thaw, add deionized water, centrifuge at 20°C, 10000r / min for 20min, discard the supernatant; obtain a precipitate; add citric acid / sesamol / Ethanol compound deodorizing agent (mass ratio of precipitation to deodorizing agent is 1:3, deodorizing agent is 0.4g sesamol and 1.0g citric acid dissolved in 100mL ethanol), stirring at low temperature for 40min, at 20°C, 8000r / min Discard the supernatant after centrifugation for 20 minutes; dry the precipitate at 55°C for sensory evaluation of fishy smell. So far, sensory evaluation is still considered to be the most convenient, efficient and effective method for fishy odor research. Human beings have the ability to detect many effective odors at extremely low concentrations, which still exceeds the sensitivity of instrumental methods in chemical analysis.

[0051] Among them, the squid ink melanin that has not been washed and deodorized is used as a ...

Embodiment 2

[0055] Thaw the squid ink sac at 0°C, soak it in the ice-water mixture overnight to thaw, add deionized water, centrifuge at 20°C, 10000r / min for 20min, discard the supernatant; obtain a precipitate; add citric acid / sesamol / Ethanol compound deodorizing agent (mass ratio of precipitation to deodorizing agent is 1:3, deodorizing agent is 0.4g sesamol and 1.0g citric acid dissolved in 100mL ethanol), stirring at low temperature for 40min, at 20°C, 8000r / min Discard the supernatant after centrifugation for 20 min; add a small amount of deionized water to the precipitated part, centrifuge at 8000 r / min at 20°C for 20 min, discard the supernatant, and repeat 8 times. Dry and freeze-dry the obtained precipitate at 50°C respectively to obtain the insoluble melanin sample; the yield of the insoluble squid ink melanin prepared by this method is 2% to 4%. Insoluble squid ink melanin carries out scanning electron microscope observation, and the result is as follows ( figure 2 ), it can...

Embodiment 3

[0057] The insoluble melanin sample obtained in Example 2 was taken and dispersed in deionized water (the ratio of precipitate to deionized water was 1.0 g of precipitate / 25 mL of deionized water). Treat in 350W ultrasonic wave for 15min, 30min, 45min, 60min, 75min, 90min respectively; after cooling to room temperature, take the mixed solution in a centrifuge tube, centrifuge at 20℃, 8000r / min for 20min, discard the precipitate, and take the supernatant to freeze The soluble melanin samples were obtained after drying, and the ultraviolet analysis was carried out on the melanin treated with different ultrasonic time ( image 3 ), it can be seen that the longer the ultrasonic time is, the higher the soluble melanin content is obtained, and the soluble melanin concentration increases slowly after the ultrasonic time exceeds 60 min. After ultrasonic treatment, insoluble squid ink melanin can be completely converted into soluble squid ink melanin. 100-500 mg of insoluble melanin c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses preparation of soluble squid ink melanin after fishy smell removal, and an application of the soluble squid ink melanin after fishy smell removal in foods, and belongs to the technical field of the foods. The preparation method comprises the following steps of unfreezing squid ink sacs, performing centrifuging, taking precipitate, then adding a citric acid/sesamol/ethanol composite fishy smell removal agent, performing stirring fishy smell removal treatment on the precipitate, then performing centrifuging, taking precipitate, performing washing repeatedly, and performing freeze-drying so as to obtain insoluble melanin samples; and dispersing the insoluble melanin in water, performing ultrasonic treatment for some time, then performing cooling, performing centrifuging, taking supernatant, and performing freeze drying so as to obtain the soluble squid melanin after fishy smell removal treatment. According to the preparation disclosed by the invention, the fishy smell removal treatment is performed through the citric acid/sesamol/ethanol composite fishy smell removal agent, so that the problem of fetor of cephalopoda melanin is thoroughly solved, and the prepared squid soluble melanin does not have any fetor taste and can be applied to development of foods, medicines and cosmetics.

Description

technical field [0001] The invention relates to the preparation of deodorized soluble squid ink melanin and its application in food, belonging to the technical field of food. Background technique [0002] Squid is a common name for squid and squid among cephalopod marine animals. Squid ink melanin is a kind of natural melanin that exists in the ink sac of cephalopod animals such as squid and squid. It is separated from ink and is insoluble in water and most liquid media. It is a chemically stable polymer. North Pacific squid is one of the most important squid processing species, with an annual processing volume of 150,000 to 300,000 tons, and its ink sac accounts for about 1.3% of the total body weight. However, the cephalopod ink sac, which is the main source of squid ink melanin, generally It is regarded as the leftovers of cephalopod processing and is discarded or processed into low-value feed, which is not well utilized, resulting in a huge waste of resources and a lot ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L5/43A23L5/20
CPCA23V2002/00A23V2200/02
Inventor 王皓芸方正胡锦华侯洁徐博学马睿金跃杰钟芸郭宏慧傅爱雯
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap