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The function of p45071d8 affecting rice plant architecture and salt stress tolerance and its application

A rice and plant technology, applied in the biological field, can solve problems such as yield reduction, and achieve the effects of enhanced stress resistance and reduced plant height

Active Publication Date: 2020-03-31
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rice is a salt-sensitive crop, and low-salt stress shows symptoms of damage, which eventually leads to significant yield reduction

Method used

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  • The function of p45071d8 affecting rice plant architecture and salt stress tolerance and its application
  • The function of p45071d8 affecting rice plant architecture and salt stress tolerance and its application
  • The function of p45071d8 affecting rice plant architecture and salt stress tolerance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Creation and identification of transgenic rice materials

[0020] 1. Construction of P450 71D8 gene recombinant plant expression vector

[0021] Using the sequence of the coding region of P450 71D8 (gene number: Os09g0483600) as a template, use primers P450 71D8-F5'-AGTGGATCC ATGTTCCCCCCAAAAATAAAAG-3', P450 71D8-R 5'-GG CGGTACCCTATAAGACAGTTGACAAAG-3' to PCR amplify P450 71D8 from the cDNA library For the target fragment with a full length of 1617bp (sequence shown in SEQ ID NO: 1), BamHI and KpnI were used to double-digest the PCR fragment and the plant expression vector pCAMBIA1205, and the digested product was ligated for 16 hours with T4 DNA ligase, and then The vector plasmid was transformed into E. coli DH5α through 42-degree heat shock transformation, and positive clones were picked for sequencing analysis to obtain the plant expression vector pCAMBIA-P450 71D8 of the P450 71D8 gene, which was introduced into Agrobacterium AGL0. The callus of Koshihika...

Embodiment 2

[0033] Example 2: P450 71D8 affects rice plant height and leaf shape

[0034] The inventors found that the plant height of rice overexpressed by P450 71D8 was reduced, and the transgenic rice overexpressed by P450 71D8 was lower than that of non-transgenic plants from the seedling stage ( figure 2 A), at the tillering stage ( figure 2 A) and heading date are significantly lower than wild-type rice, and the height is only about 60% of non-transgenic rice ( figure 2 C), In addition, at maturity, the upper first leaf (flag leaf) of transgenic rice was significantly wider and shorter than that of non-transgenic rice ( figure 2 D), These changes in rice plant height and leaf shape indicate that P450 71D8 is involved in the regulation of rice plant height and leaf shape. The transgenic application of this gene can improve rice plant height and leaf shape, and has a good application in rice production prospect.

Embodiment 3

[0035] Example 3: Overexpression of P450 71D8 improves the salt tolerance of rice

[0036] The present inventors treated non-transgenic (WT) Koshihikari and transgenic P450 71D8 rice materials for 1 week with 1% NaCl solution for 6 days, then removed the salt water, resumed normal watering and cultured for 4 days, and observed the differences between transgenic rice and wild rice after treatment. Salt tolerance differences among different types of rice, the results are as follows image 3 As shown, after 4 days of salt treatment and restoration of water supply, the wild-type plants (WT) were almost all yellow and died, while the transgenic rice overexpressed with P450 71D8 could survive, most of the plants could grow normally after salt treatment, and the leaves remained green Fresh and tender, indicating that overexpression of P450 71D8 can improve the salt tolerance of transgenic rice.

[0037] Institute of Biotechnology, Chinese Academy of Agricultural Sciences

[0038] ...

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Abstract

The invention discloses application of a paddy rice P450 family member P45071D8 in the regulation of rice plant types, leaf types and stress tolerance. P45071D8 genetically modified rice is created through the transgenic technology, the plant heights, leaf types and salt stress tolerance of the genetically modified rice are changed, and accordingly the P45071D8 gene has a good application value in rice plant type and salt stress tolerance improvement.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene for regulating rice plant height, leaf shape and salt stress tolerance and its application in the transgenic improvement of crops. Background technique [0002] Rice plant type and leaf type are one of the important factors that determine rice yield. They have long been an important goal of many crop breeding and improvement, and their regulatory mechanisms have attracted much attention. Plant height is a key factor affecting rice yield, and plant height exceeding a certain range will easily cause stem lodging and yield reduction. The breeding and popularization of rice semi-dwarf varieties in the 1950s greatly increased the yield of rice. This first "green revolution" in rice production was attributed to the application of the semi-dwarf gene sd1. In the 1970s, cross breeding using semi-dwarf materials as parents achieved the second leap in rice yield. Therefore, plant type i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/82A01H5/00A01H6/46
CPCC12N9/0071C12N15/8261C12N15/8273
Inventor 张海文张执金权瑞党王娟黄荣峰
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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