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Methods and kits for preparing biological constructs

A kit and construct technology, applied in the field of 3D bioprinting, can solve the problem of inability to achieve precise and orderly assembly of microstructures and microtissues, inability to achieve precise arrangement of cells, and inability to precisely control the spatial position and arrangement of microstructures, etc. question

Active Publication Date: 2020-09-04
SICHUAN REVOTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above two methods for constructing large-scale biological structures or tissues all have deficiencies.
For example, in the method reported by Y.Du et al., due to the spontaneous nature of the hydrophobic interaction, the spatial position and arrangement of the microstructures cannot be precisely controlled, so that the orderly assembly of the microstructures and the precise arrangement of cells cannot be achieved. cloth
In addition, in the method reported by Y.Du et al., the obtained structure must be kept stable through photocrosslinking, and the timeliness of the whole method is poor
In the method reported by Depeng Kou et al., in order to obtain large-scale biological structures or tissues, the cell culture process must be performed, and the timeliness is poor
In addition, in the method reported by Depeng Kou et al., the microstructures are in a disordered state during the cultivation and accumulation process, and the spatial position and arrangement of the microstructures cannot be precisely controlled, so that the ordered assembly of the microstructures cannot be realized, and the Precise arrangement of cells
[0006] Therefore, none of the existing methods for constructing large-scale biological structures or tissues can achieve precise and ordered assembly of microstructures and microtissues, and cannot quickly and accurately construct complex structures or tissues with precise cell arrangements (such as artificial tissues or organs)

Method used

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  • Methods and kits for preparing biological constructs
  • Methods and kits for preparing biological constructs
  • Methods and kits for preparing biological constructs

Examples

Experimental program
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preparation example Construction

[0203] The methods of the present invention for preparing biological constructs can be implemented by bioprinting methods. In certain preferred embodiments, bioprinting is performed using a printer (eg, a 3D bioprinter). In certain preferred embodiments, bioprinting is performed using automated or non-automated mechanical processes. In certain preferred embodiments, bioprinting is performed using manual placement or manual deposition (eg, using a pipette). In certain preferred embodiments, the building blocks are printed by extrusion printing or modular printing. Preferably, the structural units are printed using a modular printing method. As used herein, "modular printing method" refers to a method of printing by absorbing / grabbing modules (such as the structural units of the present invention, such as bio-bricks) and precisely positioning / arranging them. Since the structural units used in the present invention comprise cells, this kind of modular printing method is also r...

specific Embodiment approach

[0244] The invention will now be described with reference to the following examples, which are intended to illustrate the invention, but not to limit it.

[0245] Reagents, kits or instruments whose sources are not specified in the examples are all conventional products commercially available on the market. Those skilled in the art understand that the examples describe the present invention by way of example and are not intended to limit the scope of the claimed invention.

Embodiment 1

[0246] Example 1. Preparation of three-dimensional constructs using biobricks, fibrinogen and thrombin

[0247] In this example, the coagulation reaction of fibrinogen and thrombin was used to realize the rapid connection and assembly of bio-bricks, and thus a preset three-dimensional construct was prepared.

[0248] Experimental materials: bio-bricks (prepared according to the method described in Chinese patent application 201610211570.4), fibrinogen (bovine source), thrombin (bovine source), normal saline (medical grade), CaCl 2 , sterile water, and gelatin (porcine source);

[0249] Wherein, the biobrick contains mouse bone marrow mesenchymal stem cells, and its core layer contains collagen, and the shell layer contains sodium alginate; and its preparation method is as described in Chinese application 201610211570.4.

[0250] Experimental steps:

[0251] 1. Preparation of fibrinogen solution (5wt%): Weigh 0.1 g of fibrinogen and dissolve it in 2 mL of physiological saline...

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Abstract

The invention relates to a kit for preparing biological constructs and purpose thereof. The kit contains a first reagent and a second reagent which are separated from each other. The first reagent contains a first component. The second reagent contains a second component. When the first component contacts with the second component, an adhesion effect can be generated so as to realize an adhesive action. In addition, the invention also relates to a method for constructing biological structures or tissues. The method includes the usage of the above kit.

Description

technical field [0001] The invention relates to the fields of 3D bioprinting and tissue engineering. In particular, the present invention relates to a kit for the preparation of a biological construct comprising a first reagent and a second reagent separated from each other, wherein the first reagent contains a first component , the second agent contains a second component, and when the first component is in contact with the second component, it can produce an adhesion effect and realize an adhesion effect. Furthermore, the invention also relates to a method for constructing a biological structure or tissue comprising the use of the kit of the invention. Background technique [0002] In human tissues, cells are arranged and distributed according to certain rules and orders. Tissues with different functions have different cell arrangements. For example, epithelial cells that make up epithelial tissue are closely arranged in a single layer to ensure their protective functio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/22A61L27/20A61L27/54A61L27/50C12Q1/02C12N5/071B33Y70/10A61K49/00
CPCA61K49/0004A61L27/20A61L27/225A61L27/227A61L27/3834A61L27/50A61L27/54B33Y70/00C12N5/0697C12N2513/00G01N33/5008G01N2500/10C08L5/04C08L89/00
Inventor 康裕建杜明春左潇张震
Owner SICHUAN REVOTEK CO LTD
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