Method for reducing CO2 flooding minimum miscible pressure by using microbial hydrocarbon degrading bacteria
A technology of miscible pressure and hydrocarbon-reducing bacteria is applied in the field of reducing the minimum miscible pressure of CO2 flooding by using microbial hydrocarbon-reducing bacteria. Easy to apply effects
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Embodiment 1
[0038] 1) First, cultivate the seeds of YM8 depressant bacteria in a small seed bottle. The specific conditions are: culture in a shaker at a temperature of 35°C and a rotation speed of 120 rpm for about 18 hours; then in a large seed bottle The specific conditions are: culture in a shaker at a temperature of 35°C and a rotation speed of 75 rpm, and the culture time is about 16 hours;
[0039] Among them, the medium (g / L) in the small seed bottle and the large seed bottle: sucrose 10.0, corn steep liquor 5.0, disodium hydrogen phosphate 1.0, potassium dihydrogen phosphate 0.25, sodium nitrate 1.5, magnesium sulfate 0.5, sodium chloride 3.0 , pH 7.2;
[0040] 2) Then, use the pressure difference to insert the seeds into the first-level seed tank for fermentation. The specific conditions are: the temperature is 35°C, the stirring speed is 150 rpm, and the cultivation time is about 24 hours to reach the required index; Put the seeds into the secondary seed tank for fermentation,...
Embodiment 2
[0045] 1) First, cultivate the seeds of YM8 depressant bacteria in a small seed bottle. The specific conditions are: culture in a shaker at a temperature of 34°C and a rotation speed of 110 rpm for about 19 hours; then in a large seed bottle The specific conditions are as follows: culture in a shaker at a temperature of 34°C and a rotation speed of 70 rpm, and the culture time is about 15 hours;
[0046] Among them, the medium (g / L) in the small seed bottle and the large seed bottle: sucrose 15.0, corn steep liquor 7.0, disodium hydrogen phosphate 0.7, potassium dihydrogen phosphate 0.4, sodium nitrate 2.0, magnesium sulfate 0.2, sodium chloride 4.0 , pH 7.3;
[0047] 2) Then, use the pressure difference to insert the seeds into the first-level seed tank for fermentation. The specific conditions are: the temperature is 34°C, the stirring speed is 160 rpm, and the cultivation time is about 23 hours to reach the required index; Put the seeds into the secondary seed tank for fer...
Embodiment 3
[0052] 1) First, cultivate the seeds of YM8 depressant bacteria in a small seed bottle. The specific conditions are: culture in a shaker at a temperature of 36°C and a rotation speed of 130 rpm for about 20 hours; then in a large seed bottle The specific conditions are as follows: culture in a shaker at a temperature of 36°C and a rotation speed of 80 rpm, and the culture time is about 17 hours;
[0053] Among them, the medium (g / L) in the small seed bottle and the large seed bottle: sucrose 12.0, corn steep liquor 10.0, disodium hydrogen phosphate 0.5, potassium dihydrogen phosphate 0.5, sodium nitrate 1.8, magnesium sulfate 0.3, sodium chloride 5.0 , pH 7.4;
[0054] 2) Then, use the pressure difference to insert the seeds into the first-level seed tank for fermentation. The specific conditions are: the temperature is 36°C, the stirring speed is 140 rpm, and the cultivation time is about 25 hours to reach the required index; Put the seeds into the secondary seed tank for fe...
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