Skin epidermal cell viability accelerant and application thereof to skin external application agent
A technology for skin external preparations and solvents, which is applied in skin epidermal cell vitality promoters and its application in skin external preparations, and can solve problems that do not involve the viability of osmanthus fragrans epidermal cells, the effect of mitochondrial activity, etc.
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Embodiment 1
[0044] Embodiment 1: the preparation of sweet-scented osmanthus extract
[0045] Osmanthus fragrans coarse powder→25 times deionized water, extraction at 90~95°C→centrifugation (8000prm, 15min)→tubular membrane (molecular weight: 300,000) ultrafiltration→RO membrane concentration→spray drying→collect the extract powder to obtain osmanthus fragrans extract things. Solid content 100%
Embodiment 2
[0046] Embodiment 2: the preparation of sweet-scented osmanthus extract
[0047] Osmanthus fragrans coarse powder → 70% ethanol (volume concentration) ultrasonic extraction at room temperature twice (12 times, 10 times) → filter → filtrate concentrated under reduced pressure → quantify to 1 times the crude drug quality to obtain the osmanthus fragrans extract. The crude drug concentration is 1g / g, and the solid content is 31.16%.
Embodiment 3
[0048] Embodiment 3: the preparation of sweet-scented osmanthus extract
[0049] Osmanthus fragrans coarse powder → 10 times 70% ethanol (volume concentration) leaching at room temperature twice → filtration → filtrate concentrated under reduced pressure → filtrate refrigerated centrifugation (8000prm, 15min, 4°C) → supernatant quantified to 1.02 times the quality of the crude drug, to obtain Osmanthus Extract. The crude drug concentration is 0.98g / g, and the solid content is 23.39%.
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