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Preparation method for gold nanoflower with high stability

A gold nanoflower and high stability technology, which is applied in the field of nanomaterial preparation, can solve the problems of protein and other molecular denaturation, precipitation, poor monodispersity, uncontrollable experimental results, etc., and achieve strong aging resistance and high stability. Effect

Active Publication Date: 2017-12-12
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When this method synthesizes stable Jinhua particles, the incubation time of chloroauric acid and protective agent is very important. If the incubation time is too short, the colloidal stability of Jinhua particles is difficult to guarantee.
For example, people such as Nivedita added bovine serum albumin (BSA) and chloroauric acid and ascorbic acid to the reaction system at the same time, and basically did not incubate. Although flower-shaped gold nanoparticles can also be obtained, there are too many free BSA molecules, which is easy to cause Gold nanoparticles coagulate, monodispersity becomes poor, and the branch length and size of gold nanoflowers cannot be effectively regulated; and if the incubation time is too long, it is likely to cause molecular denaturation and precipitation of proteins, which will also lead to gold flowers Particle stability and monodispersity become poor, and the experimental results are uncontrollable

Method used

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  • Preparation method for gold nanoflower with high stability
  • Preparation method for gold nanoflower with high stability
  • Preparation method for gold nanoflower with high stability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Preparation of highly stable gold nanoflowers

[0037] Prepare 5mL HAuCl 4 A mixed solution with a concentration of 0.25 mM and a BSA concentration of 2.0 μM was incubated for 60 minutes, then 50 μL of 0.125 M AA was added under stirring at 300 rpm, and the pH value of the mixed solution was adjusted to 3-5 with 1 M HCl or 1 M NaOH. After reacting at room temperature for half an hour, gold nanoflowers with a branch length of 20nm and a size of 60nm were obtained. The TEM image is as follows figure 1 As shown; the obtained gold nanoflowers are very stable, and there is no obvious change in color after three months, and the spectra and photos are as follows figure 2 shown.

Embodiment 2

[0038] Example 2: Comparative experiment on stability and gold nanoflowers prepared by prior art

[0039] According to common knowledge in the field, the stability of the prepared gold nanoflowers can be improved after being coated with BSA. Prepare gold nanoflowers and coat BSA according to the method of patent CN 1817523A: take 20mL of 0.0016% HAuC1 4 solution, adjust HAuC1 with 1M NaOH solution 4 The pH value of the solution is 10, add 60 μL of 10.04M hydroxylamine hydrochloride solution, and add 2.5×10 -4 1.5mL of gold seeds with a diameter of M-25nm was stirred and reacted at 25°C for 5 minutes to obtain gold nanoflowers with a diameter of about 40nm. Take 4mL of gold nanoflowers, add 1mL of 5mg / mL BSA under stirring conditions, and act for 5h to obtain BSA-coated gold nanoflowers.

[0040] Take 4mL of the post-treatment-coated BSA-coated gold nanoflowers and the gold nanoflowers prepared in Example 1 of the present invention respectively, and then add 1mL of 2M NaCl s...

Embodiment 3

[0042] Example 3 Controlling the branch length of gold nanoflowers by changing the incubation time

[0043] The incubation time in Example 1 was changed from 60 minutes to 40 minutes and 90 minutes respectively, and other conditions remained unchanged, and gold nanoflowers with branch lengths of 10nm and 23nm were finally obtained, and the TEM images of the gold nanoflowers obtained were as follows: Figure 5 and Figure 6 As shown; the obtained gold nanoflowers are very stable, and the color has no obvious change after three months, and the spectra and solution photos are shown in Fig. Figure 7 and Figure 8 shown.

[0044] It can be seen from this example that the present invention can regulate the branch length of gold nanoflowers by changing the incubation time.

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Abstract

The invention discloses a preparation method for a gold nanoflower with high stability, and belongs to the technical field of nano material preparation. The preparation method comprises the following steps of: taking blood albumin as a morphology inducer, taking ascorbic acid as a reducing agent, and taking chloroauric acid as a gold precursor; mixing chloroauric acid with blood albumin, and adding the reducing agent after hatching for 40-90 minutes; and reacting for half one hour under conditions with a pH value of 3-5 and a temperature of 4-30 DEG C, thereby obtaining the gold nanoflower with high stability. The gold nanoflower prepared by the preparation method has the advantages of high stability, strong salt and curing resistance, adjustable branch length, an adjustable dimension and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation, and specifically relates to a highly stable gold nanometer with adjustable branch length and size, which uses serum protein as a shape inducer, ascorbic acid as a reducing agent, and chloroauric acid as a gold precursor. The way to spend. technical background [0002] Gold nanoflowers have unique optical properties due to their multi-branched structure and rough surface, and have broad application prospects in the fields of surface-enhanced Raman scattering, biomarkers and detection, and photothermal therapy. The optical properties of gold nanoflowers strongly depend on their branch length and particle size, and gold nanoflowers with different branch lengths and sizes have different optical effects. Therefore, the adjustable branch length and particle size can obviously meet the needs of a wider range of applications. The synthesis methods of gold nanoflowers are mainly divided...

Claims

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Application Information

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IPC IPC(8): B22F9/24B82Y40/00
CPCB82Y40/00B22F9/24B22F1/0553
Inventor 张小玉韩延东杨文胜
Owner JILIN UNIV
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