Enzyme catalyzed organic chemical reaction in redox stimulus responsive Pickering emulsion
A stimuli-response and emulsion technology, applied in the field of physical chemistry, can solve the problems of low recycling rate, low reaction efficiency, difficult enzyme recovery, etc., and achieve the effect of enhancing contact opportunities
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[0022] Example 1: Nano SiO 2 Pickering emulsion prepared by granules, FTMA and lipase. In a series of cylindrical bottles with a volume of 25 mL, accurately weigh the nano SiO with a mass fraction of 0.5% (relative to the water phase) 2 , Add 7mL FTMA solution of different concentrations. Disperse the particles with an ultrasonic disperser (90W, 15s). After the nanoparticles are evenly dispersed, add a certain amount of lipase solution (the final concentration of lipase in the water phase is 1 mg·mL) -1 ) And 7mL isooctane, emulsify with a homogenizer (IKA ULTRA-TURRAX T18basic) at a homogenization rate of 11000rpm for 2min to obtain an O / W Pickering emulsion. After the emulsion is placed, take a picture of its appearance, such as figure 2 with image 3 Shown.
Example Embodiment
[0023] Example 2: Pickering emulsion prepared by nano calcium carbonate particles, FTMA and reductase. Take 7mL of a series of surfactant aqueous solutions with a series of concentrations into a 25mL cylindrical vial, add 0.03g commercial nano calcium carbonate particles (the primary particle size is about 10nm, the BET specific surface area is 250m 2 / g), use an ultrasonic disperser to disperse the particles (50W, 1min), add 7mL n-heptane and a certain amount of reductase solution (the final concentration of reductase in the water phase is 1mg·mL -1 ), emulsify with a high-shear emulsifier for 2 min to obtain a stable O / W Pickering emulsion.
Example Embodiment
[0024] Example 3: Pickering emulsion prepared by nano titanium dioxide particles, FTMA and transferase. Take 7 mL of a series of surfactant aqueous solutions with a series of concentrations into 25 mL cylindrical vials, and add 0.03 g of commercial nano titanium dioxide particles (the primary particle size is about 20nm, the BET specific surface area is 300m 2 / g), use an ultrasonic disperser to disperse the particles (50W, 1min), add 7mL isooctane and a certain amount of transferase solution (the final concentration of reductase in the water phase is 1.5mg·mL -1 ), emulsify with a high-shear emulsifier for 2 minutes to obtain a stable O / W Pickering emulsion.
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