Multilayer Conjugate Aberration Correction System and Method Based on Parallel Adaptive Optics Correction

A technology of adaptive optics and aberration correction, applied in optics, optical components, instruments, etc., to achieve the effect of large depth imaging

Active Publication Date: 2020-04-24
ZHEJIANG UNIV
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Problems solved by technology

[0004] In order to solve the depth imaging problem existing in the background technology, the present invention provides a multilayer conjugate aberration correction system and method based on parallel adaptive optics correction

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  • Multilayer Conjugate Aberration Correction System and Method Based on Parallel Adaptive Optics Correction

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Embodiment Construction

[0046] The present invention will be further described below in conjunction with drawings and embodiments.

[0047] The system of the present invention includes a first lens L1, a second spatial light modulator SLM2, a second lens L2, a third lens L3, a first spatial light modulator SLM1, a fourth lens L4 and an objective lens L5 arranged in sequence along the optical path direction, There is a scattering medium in front of the objective lens L5, and the scattering medium includes two layers of different thicknesses, the first half layer and the second half layer, the first half layer is closer to the objective lens L5 than the second half layer; the incident light is sequentially modulated by the first lens L1 and the second spatial light SLM2, the second lens L2, the third lens L3, the first spatial light modulator SLM1, the fourth lens L4 and the objective lens L5 are then focused onto the rear end surface of the scattering medium, and the rear end surface of the scattering ...

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Abstract

The invention discloses a multi-layer conjugate aberration correction system and method based on adaptive optical correction. The multi-layer conjugate aberration correction system and method based on adaptive optical correction utilize a plurality of spatial light modulators and utilize a four-focal-length system to optically conjugate the spatial light modulators to each layer of a scattering medium, wherein each spatial light modulator is responsible for correcting the corresponding layer, and successively performs one time of parallel adaptive optical correction algorithm, and then the scattering medium is corrected through gradual conjugation from shallow to deep, and finally light spot correction of the scattering medium with relatively high thickness can be realized. The multi-layer conjugate aberration correction system and method based on adaptive optical correction can realize aberration correction of the deep layer of scattering medium to enable the imaging depth to be deeper, and can break through limitation of traditional adaptive optical imaging depth.

Description

technical field [0001] The invention belongs to a deep scattering medium imaging method in the field of microscopic imaging, and in particular relates to a multilayer conjugate aberration correction system and method based on parallel adaptive optics correction. Background technique [0002] In the field of biomedical research, the light microscope is an indispensable research tool. But when we observe some biological samples (such as cells, subcellular organelles, blood vessels, etc.), the non-uniformity of the refractive index of these biological tissues will cause the incident light to scatter, so that the imaging range is limited to shallow layers, making in vivo imaging extremely challenging. sex. But in the field of biomedical microscopy, the information contained in deep tissues is what we want to explore more, so in order to obtain deeper information, correcting and focusing the light inside the turbid medium has become a long-standing goal. In order to achieve thi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B27/00G02B21/36
CPCG02B21/361G02B27/0025
Inventor 斯科龚薇章一叶
Owner ZHEJIANG UNIV
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