Method for measuring aquatic organism intestinal tract pH (potential of hydrogen) based on nano-fluorescent dyes

Abstract

The invention relates to a method for measuring aquatic organism intestinal tract pH (potential of hydrogen) based on nano-fluorescent dyes. The method includes the steps: (1) dissolving dioleoylphosphocholine (DOPC) in trichloromethane solution to prepare storing solution, and adding the storing solution into a glass bottle; adding daphnia magna culture solution into the glass bottle; adding nano-fluorescent dyes of 10 micrometers into the daphnia magna culture solution; measuring fluorescent characteristics of TPE-Cy under different pH conditions by the aid of a fluorescent instrument; scanning daphnia magna intestinal tracts by the aid of a confocal laser scanning microscope, and analyzing overall fluorescence of the daphnia magna intestinal tracts. According to the method, overall pH distribution of the daphnia magna intestinal tracts is measured by the nano-fluorescent dyes, the method is simple, convenient and rapid to operate and high in sensitivity, and measuring accuracy is greatly improved.

Description

technical field [0001] The invention belongs to the field of environmental analytical chemistry, and relates to a method for measuring the intestinal pH of aquatic organisms based on nanometer fluorescent dyes, which is used for measuring the pH in the intestinal tract of Daphnia magna. Background technique [0002] Daphnia magna, as a common aquatic organism, is widely distributed in lakes and rivers. Due to its easy breeding, short life cycle and special translucent body structure, daphnia magna has become a model organism widely used in toxicology, genomics and other research. In recent years, the gut of Daphnia magna has attracted much attention. The intestine is the main excretory organ of Daphnia magna. After a large amount of food is ingested, it will stay in the intestine for a long time. Dao plays an important role in the life activities of Daphnia magna. The pH in the intestinal tract has a direct impact on the activity of digestive enzymes, and ultimately affec...

AI Technical Summary

Problems solved by technology

However, both techniques have certain limitations

Microelectrode technology cannot realize the non-destructive detection of the living body, and it will cause fatal damage to the organism during the operation. Determination of channel pH distribution

On the contrary, fluorescent technology can realize the determination of the pH distribution of the whole intestinal tract of zooplankton, but the biocompatibility of traditional fluorescent materials is poor, so it will cause a certain degree of toxicity to organisms

In addition, traditional fluorescent dyes have poor sensitivity and relatively low accuracy, and prolonged excitation may lead to fluorescence quenching

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