Novel biomarkers for susceptibility prediction associated with epidermal growth factor receptor-target agents and uses thereof
A use and receptor technology, applied in the field of new biomarkers for sensitivity prediction related to epidermal growth factor receptor-target preparations and its use, can solve the problem of weak results, and it is difficult to ensure a wide range of therapeutic agents and administration methods. Such problems, to achieve the effect of avoiding side effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0188] Example 1. Activity of Epidermal Growth Factor Receptor Based on RON Protein Viability
[0189] 1-1. Existence of active form of RON protein in human colorectal cancer cell lines
[0190] The present inventors confirmed RON phosphorylation in a total of 19 human colorectal cancer cell lines by Western blotting in order to screen colorectal cancer cell lines in which RON protein was activated or inactive.
[0191] This phosphorylated RON protein is considered as RON active protein.
[0192] As a result, such as Figure 1a As shown, among the 18 types of cell lines, RON phosphorylated protein expression was observed in 4 cell lines (HT-29, KM12C, KM12L4, and SW1417), and it was found that 4 cell lines have RON activity.
[0193] On the other hand, in LoVo and Colo320HSR colorectal cancer cell lines, RON protein itself does not appear.
[0194] 1-2. Gene expression analysis based on RON protein activation by DNA microarray analysis
[0195] In order to analyze the gene ...
Embodiment 2
[0210] Example 2. Changes in human epidermal growth factor receptor activity based on whether RON activity is present
[0211] 2-1. Activation of epidermal growth factor receptor protein based on activation of RON protein
[0212] In order to confirm the activation of epidermal growth factor receptor protein by RON activation, the present inventors overexpressed the Δ160 gene, which is the active form of the RON gene, in the LoVo and Colo320HSR colorectal cancer cell lines that do not express RON, and then performed protein analysis. Blotting.
[0213] As a result, such as Figure 2a As shown, it was confirmed that the activity of activating the epidermal growth factor receptor by RON was increased.
[0214] 2-2. Changes in activity of epidermal growth factor receptor protein due to RON inhibition
[0215] In order to confirm the change in the activity of the epidermal growth factor receptor protein due to the activation of the RON protein, the present inventors artificiall...
Embodiment 3
[0220] Example 3. Drug sensitivity analysis of cetuximab based on activation of RON protein
[0221] 3-1. Endogenous intracellular binding analysis between RON protein and epidermal growth factor receptor protein
[0222] In order to analyze the binding between RON protein and epidermal growth factor receptor protein in the CaCO2 colorectal cancer cell line in an inactive state of RON and the KM12C colorectal cancer cell line in which RON is activated, the present inventors used RON antibody to Immunoprecipitation was used to observe whether the RON protein was combined with the epidermal growth factor receptor protein.
[0223] As a result, such as Figure 3a As shown, it can be seen that the RON protein binds to the epidermal growth factor receptor protein in CaCO2 cells without RON activity, while the RON protein binds to the epidermal growth factor receptor protein in KM12C cells with RON activity .
[0224] 3-2. Analysis of exogenous intracellular binding between RON p...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More