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A technology of Stenotrophomonas maltophilia, which is applied in the field of strains and Stenotrophomonas maltophilia, to achieve the effect of increasing the aroma of tobacco leaves and strong diffusivity
Active Publication Date: 2019-12-10
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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[0006] The present invention provides a strain of Stenotrophomonas maltophilia to solve the problem of degradation of 2,7,11-Cembratriene-4,6-diol in tobacco (Stenotrophomonas maltophilia) and its application
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Embodiment 1
[0049] 1. Experiment preparation
[0050] The public formula of Gao formula No. 1 medium:
[0051] KNO 3 1.0 g / L, KH 2 PO 4 0.5g / L, NaCl 0.5g / L MgSO 4 •7H 2 O 0.5 g / L, FeSO 4 •7H 2 O0.01 g / L, add 0.3g / L 2,7,11-cembratriene-4,6-diol on the basis of this medium.
[0055] Stenotrophomonas maltophilia (Stenotrophomonas maltophilia) Inoculated into 30 mL of LB medium, cultured for 12 h and then used for later use.
[0056] 2. Detection of Bacterial Degradation Characteristics of 2,7,11-Cembratriene-4,6-diol
[0057] The seed solution of Stenotrophomonas maltophilia was inoculated into 0.3 g / L bacterial inorganic salt culture medium with 1% inoculum volume for 24 h, the temperature was 32 °C, and the shaker speed was 150 rpm. At the same time, a blank control was made. Sampling was done every 6 h,...
Embodiment 2
[0062] Stenotrophomonas maltophilia was activated and cultured according to the method of Example 1.
[0063] Application of a strain of Stenotrophomonas maltophilia as fermented reconstituted tobacco leaf concentrate. Specific steps are as follows:
[0064] 1) The formula of the medium: KNO 3 1.0 g / L, KH 2 PO 4 0.5g / L, NaCl 0.5g / L MgSO 4 •7H 2 O 0.5g / L, FeSO 4 •7H 2 O 0.01 g / L, maltose 1 g / L.
[0065] 2) Strain activation: Stenotrophomonas maltophilia H3-1 was activated and cultured to obtain Stenotrophomonas maltophilia activation solution; the strain activation conditions were: 35°C, pH 7, 150 r / min shaking culture for 72 Hour.
[0066] 3) Fermentation: Inoculate the activated bacterial liquid obtained in step 2) into the culture medium in the above step 1) according to the volume ratio of 10% to form a fermentation liquid. The fermentation culture conditions after inoculation are the same as the activation culture conditions, and the culture time is 4 days ;
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Abstract
The invention belongs to the field of bioengineering and relates to a bacterial strain, in particular to a strain of Stenotrophomonas maltophilia and its application. The Stenotrophomonas maltophilia strain was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on March 20, 2017, and its preservation number is CGMCC No.13905. The bacterium is used for degrading 2,7,11-cembratriene-4,6-diol and fermenting reconstituted tobacco leaf concentrate. During the aging of tobacco leaves, the Stenotrophomonas maltophilia of the present application can degrade 2,7,11-cembratriene-4,6-diols into aroma substances, thereby increasing the aroma of tobacco leaves. In the application of the concentrated liquid, the taste of the reconstituted tobacco leaves can be improved by improving the aroma quality and aroma quality and reducing irritation after conversion.
Description
technical field [0001] The invention belongs to the field of bioengineering and relates to a bacterial strain, in particular to a strain of Stenotrophomonas maltophilia and its application. Background technique [0002] Cembrene compounds are also called thunbergen or duvatriene compounds, chemically, the structure of this type of substance belongs to macrocyclic diterpenoids. Such substances not only exist in tobacco leaves, but also in tobacco seeds and tobacco flowers, natural plants such as turpentine, frankincense, and a few marine organisms. According to relevant literature reports, there are more than 50 kinds of cemberene compounds in tobacco. [0003] Cibardenes are an important class of flavor precursors in tobacco. Such substances are metabolized during the growth of tobacco and mainly exist in the glandular hair secretions on the surface of tobacco leaves. During the aging period of tobacco, cemberenes are mainly degraded during the conditioning process, and th...
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