Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device

A sampling device and microbial technology, which is applied in the field of complete sampling equipment and human intestinal microbial sampling, can solve the problems of the operator's biological material contact and the sampling device cannot safely and reliably sample microorganisms, and achieve the effect of increasing the survival rate

Inactive Publication Date: 2018-01-02
MAAT PHARMA +1
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Problems solved by technology

[0014] However, the known sampling devices do not provide the conditions for simply ensuring the desired preservation of microbiological bacteria, especially anaerobic bacteria, the main constituents of intestinal microbiota.
In addition, known sampling devices cannot safely and reliably perform the operations required for microbial sampling, and the operators who perform the operations are at risk of coming into contact with biological substances

Method used

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  • Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device
  • Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device
  • Microorganism sampling method, microorganism sampling device and sampling kit comprising such a sampling device

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Embodiment Construction

[0049] In the figures, the same reference numerals designate the same or similar elements.

[0050] figure 1 An embodiment of a sampling plant 1 is shown having a sampling device 2 for sampling microorganisms contained in biological matter. In the illustrated embodiment, without limitation, the sampling device 2 is used for sampling intestinal microorganisms contained in human faeces.

[0051] The sampling device 2 has a container 5 which is used in particular to collect faeces.

[0052] The container 5 has a body 6 consisting of a flexible bag defining an inner space 7 suitable for receiving biological matter and having an upper edge 8 . The body 6 is made of a deformable material suitable to form a barrier between the faeces contained in the inner space 7 and the external fluids, avoiding any exchange between the collected faeces and the outside. In particular, the body 6 may be made of polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polycarbonate (PC) or...

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Abstract

A sampling method using a sampling device (2) comprising a container (5) comprising a body (6) consisting of a soft pouch, and a lid (30), in which at least one among the container (5) and the lid (30) is provided with a discharging member (40) suitable for, in an open state, discharging at least one part of the gases contained in the internal space (7) of the body (6) of the container (5), wherein the sampling method comprises the steps consisting in: - collecting a biological material in the body (6) of the container (5), and assembling the lid (30) on the neck (10) of the container (5), - and placing an internal space (7) of the body (6) of the container (5) under anaerobic conditions by placing the discharging member (40) in the open state, by compressing the body (6) of the container(5) and by placing the discharging member (40) in a closed state.

Description

technical field [0001] The invention relates to a microbial sampling method, a microbial sampling device and a complete set of sampling equipment using the microbial sampling device. [0002] The present invention is particularly applicable to the sampling of human intestinal microbes, for example, for treating intestinal metabolic disorders, such as refractory Clostridium infection, through the transplantation of intestinal microbes. Background technique [0003] The gut microbiome consists of all microorganisms (bacteria, yeast, and fungi) in the human gastro-intestinal system (intestine, stomach, and stool). Microbial diversity is now estimated at about 10 3 bacterial species, including the gut microbes of adults, with an abundance of 10 14 bacteria, which means that each person has a bacterial genome of 200,000 to 800,000 genes, which is 10 to 50 times the number of genes in the human genome. [0004] The prenatal gut is sterile and develops ex situ from the first few...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/00
CPCA61B10/0038A61B10/0096G01N1/10G01N2001/1025
Inventor C·施文特纳H·阿法加德J·多尔
Owner MAAT PHARMA
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