L-lactic acid fermentation method

A process method, lactic acid fermentation technology, applied in the field of L-lactic acid fermentation process and plant fruit fermentation process, can solve the problems of long fermentation period, low conversion rate, unstable quality, etc., and achieve short fermentation period and high acid production rate , good effect of lactic acid purity

Inactive Publication Date: 2018-01-05
姜桂萍
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  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, low PH value requires high equipment. Most manufacturers use stainless steel SUS304, and some even use SUS316L, which increases the input cost; on the other hand, the traditional fermentation cycle is long (mostly more than 70 hours), which is easy to dye Bacteria, low lactic acid purity, low acid production (below 12%), low conversion rate, resulting in high cost
At the same time, the addition of too much nitrogen in the traditional process medium (such as a large amount of corn steeple 6% and yeast powder 4%) leads to difficulty in extraction, dark color of the finished product, and unstable quality

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0006] Firstly, the rice is crushed, then the rice is liquefied and saccharified by a specific process, and a small amount of amino acid is added to the rice, which is placed in a carbon steel tank and fermented. At the same time, an appropriate amount of calcium hydroxide is added online during the fermentation process, so that the pH value of the solution is controlled at 6.0-7.0. At the same time, due to the addition of calcium hydroxide to enhance the mobility of the reactants, through the change of the medium, the bacteria can obtain the best growth conditions and further shorten the cycle (about 30 hours); after repeated mutagenesis, the high temperature resistance / high acid production / The acid production rate is above 15% for bacteria strains with a near-neutral pH value; calcium hydroxide solution is used as an additive to control the pH around 7.0, and carbon steel tanks are used as fermentation tanks to produce L-lactic acid with a purity of more than 99%.

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PUM

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Abstract

The invention relates to an L-lactic acid fermentation process method, which is suitable for the fermentation process of plant fruits and belongs to the technical field of biochemical industry. It includes rice crushing, rice liquefaction, saccharification, adding trace amino acids and fermenting in carbon steel tanks. It is characterized in that an appropriate amount of calcium hydroxide is added online during the fermentation process; the pH value of the appropriate amount of calcium hydroxide solution is 6.0-7.0. The invention has the advantages of short fermentation period, high acid production rate, good lactic acid purity and the like.

Description

technical field [0001] The invention relates to an L-lactic acid fermentation process, which is suitable for the fermentation process of plant fruits and belongs to the technical field of biochemical industry. Background technique [0002] At present, most traditional lactic acid fermentations use regular addition of calcium carbonate as a neutralizer to control fermentation, and most of them use the form of pre-adding excess calcium carbonate. During this period, the metabolized products make the fermentation pH low, and the pH value is usually 5.0 the following. On the one hand, low PH value requires high equipment. Most manufacturers use stainless steel SUS304, and some even use SUS316L, which increases the input cost; on the other hand, the traditional fermentation cycle is long (mostly more than 70 hours), which is easy to dye Bacteria, low lactic acid purity, low acid production (below 12%), low conversion rate, resulting in high cost. At the same time, the addition ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56
Inventor 不公告发明人
Owner 姜桂萍
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