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Liquid culture method of mushrooms

A technology of liquid culture and shiitake mushrooms, which is applied in mushroom cultivation, botanical equipment and methods, cultivation, etc. It can solve the problems of troublesome management and long fruiting time, achieve the effects of shortening the fruiting time and overcoming the problem of bacterial contamination

Inactive Publication Date: 2018-01-09
翔天菌业集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional shiitake mushroom cultivation usually takes five to six months to produce mushrooms, which takes a long time; due to the long mushrooming time, it brings a lot of trouble to management, especially the problem of bacterial contamination

Method used

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  • Liquid culture method of mushrooms
  • Liquid culture method of mushrooms

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Experimental program
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Effect test

Embodiment 1

[0027] figure 1 As shown, a culture bottle includes a cylindrical body 1 and a conical end cap 2 arranged on the upper end of the cylindrical body 1; the top of the conical end cap 2 is provided with an inoculation hole 3, and the inoculation hole 3 is provided with A sealing plug 4; the cylindrical body 1 is sealed and connected with the conical end cap 2; an air intake pipe 5 and an exhaust pipe 6 are arranged in the sealing plug 3, and the air intake pipe 5 and the exhaust pipe 6 are arranged on the conical end cap 2 are provided with a microporous sealing cover 7 on the outside. The cylindrical body can be a cylindrical barrel or a rectangular cylindrical barrel; the tapered end cap is a cone or a prism corresponding to the shape of the cylindrical body. The inoculation hole at the top of the tapered end cap can facilitate the inoculation operation, and the sealing plug is convenient for sealing and maintaining the aseptic environment in the culture bottle. The air intak...

Embodiment 2

[0033] A liquid culture method for shiitake mushrooms, comprising the steps of:

[0034] a) Prepare culture solution, put the culture solution and porous floating body into the culture bottle, seal and sterilize;

[0035] The culture medium includes: 200 grams of potatoes, 10 grams of corn flour and 15 grams of oak wood chips, 1000 milliliters of water, 20 grams of sucrose, 1.5 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, and 2 grams of peptone;

[0036] The porous floating body is a foam glass plate or foam glass beads uniformly provided with through holes;

[0037] The culture flask is the culture flask described in Example 1, a large-capacity jar or an Erlenmeyer flask.

[0038] b) Under sterile conditions, insert the strains into the culture bottle and seal it.

[0039] c) The temperature is 24-27°C, and the culture is static for 10-15 days, and the mycelium is covered with the liquid surface.

[0040] d) Cultivate mushrooms at 8~16°C for 15~...

Embodiment 3

[0044] The same as Example 2, the difference is that the culture solution includes: 150 grams of potatoes, 5 grams of corn flour and 10 grams of oak wood chips and 1000 milliliters of water, 15 grams of sucrose, 1 gram of potassium dihydrogen phosphate, 0.3 grams of magnesium sulfate, peptone 1 g.

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Abstract

The invention relates to a culture method of mushrooms. The liquid culture method includes the steps of 1), preparing culture liquid, placing the culture liquid and a porous floating body into a culture bottle to be sealed, and performing sterilization; b), under the sterile condition, accessing strains into the culture bottle to be sealed; c), performing static culture for 10-15 days at 24-27DEGC till mycelium grows all over a liquid surface; d), performing fruiting culture for 15-20 days at 8-16DEG C, after sporophore grows above the liquid surface, feeding sterile air 60-70% in relative humidity to above the liquid surface for air change and performing fruiting culture; e), after 70-80 days of fruiting culture, picking the mushrooms. The liquid culture method is short in period and convenient in operation.

Description

technical field [0001] The invention relates to a liquid culture method of shiitake mushrooms. Background technique [0002] Mushrooms are known as the king of delicacies, and they are high-protein, low-fat nutritional and health food. Chinese medical scientists of all dynasties have famous expositions on shiitake mushrooms. Modern medicine and nutrition continue to in-depth research, and the medicinal value of shiitake mushrooms is also constantly being explored. [0003] The traditional cultivation method of shiitake mushrooms is culture material rod cultivation, and its cultivation raw materials, the formula and preparation method of culture materials are the same as the cultivation of shiitake mushrooms with sawdust bacteria bricks. Its cultivation season is best to start making mushroom sticks successively in late August, and the mushrooms will be produced after the beginning of winter and before the "May 1st" of the following year. The ideal place for cultivation is...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20C05G1/00
Inventor 张金霞黄晨阳肖俊培汪建党侯艳平侯晓强韩美玲王晶乔杰隋杰
Owner 翔天菌业集团股份有限公司
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