Customized skincare product prepared by bioactive factors of fetus and preparation method thereof

Abstract

The invention relates to a customized skincare product prepared by bioactive factors of fetus and a preparation method thereof, in particular relates to a method of enriching blood platelets and extracting the active factors from the fetus. The method comprises the following steps: (1) enrichment of the blood platelets; (2) preparation of blood platelet lysate: diluting a blood platelet sample enriched in the step (1) according to concentration of blood platelets; and performing chemical splitting and physical splitting to obtain the lysate; (3) preparation of concentration liquor: concentrating the supernatant after centrifugalization in the step 2 in the step of enriching the blood platelets; (4) preparation of bioactive factors of the fetus: fully mixing the lysate and the concentratedliquor; and (5) lyophilization and preservation of bioactive factors of fetus. The invention also relates to lyophilized powder. The product provided by the invention has the beneficial effects described in the description, for example, the prepared active factor mixed liquid or a cryopreserved article or lyophilized powder prepared therefrom can be directly taken as the customized skincare product or can be further processed to prepare the customized skincare product.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for enriching platelets and extracting active factors from placenta, in particular to a method for enriching platelets and extracting active factors from autologous placenta. More particularly, the present invention relates to the preparation of customized beauty and skin care products by using placental bioactive factors, and the preparation method of the customized beauty and skin care products. The active factor prepared in the present invention can be used in combination with a solvent to be directly used in beauty and skin care, including beauty treatment, freckle removal, scar repair, etc., or added to various formulations of beauty and skin care products. These preparations may include injections, face creams, lotions, water solutions, or various common preparations. The active factor prepared by the invention can also be directly applied to the damaged skin surface or i...

AI Technical Summary

Problems solved by technology

However, the liquid culture supernatant has a short storage time at room temperature, which hinders its promotion and application.

[0008] In addition, for the current stem cell sorting technology, magnetic bead sorting is more common. However, in conventional sorting, the magnetic beads will be cultured together with the stem cells, that is, the magnetic beads are not detached from the stem cells. The later production will definitely have a certain impact. Although there are some methods, such as adding some bacteria or other substances to degrade, this is equivalent to adding other exogenous proteins and other components, causing secondary pollution to stem cells, so doesn't actually solve the above problem

[0009] There are also some reports on the extraction and purification of stem cell culture supernatant, but the effect is still not good. For example, the patent with the notification number CN102600057B discloses a preparation method of human placental stem cell extract freeze-dried powder. A single 3000D filter membrane is used for ultrafiltration interception, which removes some impurities, but the impurities such as sugar are still not removed, and it also causes a large loss of cytokines, poor repair effect, and also causes discomfort such as dry skin , the practical application value is low

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