Method for enriching blood platelets from placenta and extracting active factors

Abstract

The invention relates to a method for enriching blood platelets from a placenta and extracting active factors. Specifically, the method for enriching blood platelets from the placenta and extracting active factors comprises the steps of (1) blood platelet enrichment; (2) blood platelet lysate preparation, namely a blood platelet sample enriched in the step (1) is diluted according to the concentration of blood platelets; chemical lysis is conducted: blood platelet activation is conducted according to the volume ratio of the diluted precipitate to an activator being 1:(5-10); the solution is kept still at room temperature, centrifugation is conducted, a supernate and sediment are collected respectively, and the supernate is stored in a refrigerator of 4 DEG C; physical lysis is conducted: the sediment collected in the previous step is subjected to repeated freezing and thawing for three times of lysis at 37 DEG C / -80 DEG C to obtain a lysate; and the supernate collected in the previous step and a product generated after lysis are sufficiently and evenly mixed to obtain the lysate; and (3) freeze-drying and storage of platelet-derived active factors. The invention further relates to freeze-dried powder. The method has the beneficial effects as described in the description.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for enriching platelets and extracting active factors from placenta, in particular to a method for enriching platelets and extracting active factors from autologous placenta. The active factor prepared in the present invention can be used in combination with a solvent to be directly used in beauty and skin care, including beauty treatment, freckle removal, scar repair, etc., or added to various formulations of beauty and skin care products. These preparations may include injections, face creams, lotions, water solutions, or various common preparations. The active factor prepared by the invention can also be directly applied to the damaged skin surface or injected with microneedles for wound healing. Background technique [0002] In recent years, with the deepening of research on mesenchymal stem cells, its secreted factors have become a hot spot for researchers. Mesenchymal ...

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Problems solved by technology

However, the liquid culture supernatant has a short storage time at room temperature, which hinders its promotion and application.

[0008] In addition, for the current stem cell sorting technology, magnetic bead sorting is more common. However, in conventional sorting, the magnetic beads will be cultured together with the stem cells, that is, the magnetic beads are not detached from the stem cells. The later production will definitely have a certain impact. Although there are some methods, such as adding some bacteria or other substances to degrade, this is equivalent to adding other exogenous proteins and other components, causing secondary pollution to stem cells, so doesn't actually solve the above problem

[0009] There are also some reports on the extraction and purification of stem cell culture supernatant, but the effect is still not good. For example, the patent with the notification number CN102600057B discloses a preparation method of human placental stem cell extract freeze-dried powder. A single 3000D filter membrane is used for ultrafiltration interception, which removes some impurities, but the impurities such as sugar are still not removed, and it also causes a large loss of cytokines, poor repair effect, and also causes discomfort such as dry skin , the practical application value is low

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