Gene expression profile detection kit for predicting breast cancer reoccurrence of Chinese population

A technology for detecting kits and gene expression profiles, applied in the field of medical molecular biology, to achieve good clinical application prospects and the effect of preventing recurrence

Active Publication Date: 2018-01-12
JIANGSU CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the breast cancer gene spectrum used to guide the treatment of breast cancer and predict breast cancer is mainly the research results of European and American countries. There

Method used

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  • Gene expression profile detection kit for predicting breast cancer reoccurrence of Chinese population
  • Gene expression profile detection kit for predicting breast cancer reoccurrence of Chinese population
  • Gene expression profile detection kit for predicting breast cancer reoccurrence of Chinese population

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) extraction of breast cancer paraffin specimen mRNA: (Ambion company RecoverALL was adopted TM Total Nucleic Acid Isolation Kit)

[0013] 1. Cut 5-6 10μm wax slices with a paraffin microtome and put them in a 1.5mL RNA free centrifuge tube.

[0014] 2. Add 1mL xylene for dewaxing, 50°C water bath, 3-5min, 12000rpm, 2min, discard xylene.

[0015] 3. Add 1mL of 100% ethanol, 12000rpm, 2min, discard the ethanol, repeat the ethanol wash once (if the dewaxing is not complete, add xylene and repeat the above steps).

[0016] 4. After blotted with ethanol, air dry for 20 minutes, add 100 μL of digestion solution and 4 μL of protease.

[0017] 5. After mixing, 50°C, 15min; 80°C, 15min.

[0018] 6. Add 120 μL of Isolation additive and 275 μL of 100% ethanol, mix up and down.

[0019] 7. Put the filter element into the collection tube, add the above mixed solution into the filter element, 10000rpm, 30s.

[0020] 8. After discarding the waste liquid, add 700μL wash1 to the...

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Abstract

The invention discloses a characteristic gene expression profile applied to predicting breast cancer reoccurrence of Chinese population and also provides a recurrence index calculation formula and a recurrence prediction judgment standard. The invention further provides a fluorescent quantitative detection kit for the breast cancer characteristic gene profile. A PCR (Polymerase Chain Reaction) amplification primer disclosed by the invention is composed of eight pairs of primers, and the following eight gene correlated expressions can be subjected to combined detection: CCNA2, CCND1, SERP1NE1,SPRR1B, TOP2A, beta-ACTIN, GAPDH and HPRT1. The characteristic gene expression profile disclosed by the invention can be used for guiding individualized treatment of clinical breast cancer and preventing breast cancer reoccurrence.

Description

technical field [0001] The invention belongs to the field of medical molecular biology, and in particular relates to a gene expression spectrum detection kit for predicting recurrence of breast cancer in Chinese population. Background technique [0002] Breast cancer is a common malignant tumor in women, and it is the malignant tumor with the highest morbidity and mortality among women in the world. Statistics released by the China Anti-Cancer Association show that the incidence of breast cancer in my country has been increasing at an annual rate of 3% in recent years, and the age of onset is also gradually becoming younger. Treatments for breast cancer include surgery, radiation therapy, chemotherapy, endocrine therapy, and biological therapy. Conventional treatment is surgery to remove tumor lesions as much as possible, and postoperative radiotherapy and chemotherapy to kill residual tumor cells and prevent tumor recurrence. Chemotherapy is an effective means of treatmen...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
Inventor 马蓉吴建中曹海霞冯继锋井昶雯王卓
Owner JIANGSU CANCER HOSPITAL
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