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Acid cellulase preparation for low-temperature biological polishing and preparation method thereof

A technology of acid cellulase and bio-polishing, which is applied in biochemical fiber treatment, fiber treatment, textiles and papermaking, etc. It can solve problems such as air pollution, achieve the effects of reduced usage, small strong damage, and high-efficiency polishing effect

Active Publication Date: 2018-01-16
FIBER CHEM BIOLOGICAL CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, coal was mainly burned, and the combustion of coal will produce a large amount of SO 2 , CO 2 and soot and other pollutants, causing serious air pollution

Method used

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  • Acid cellulase preparation for low-temperature biological polishing and preparation method thereof
  • Acid cellulase preparation for low-temperature biological polishing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Determination of enzyme activity

[0035] 1.1 Enzyme activity determination method: under the conditions of 50°C and pH=4.8, the amount of enzyme required to degrade and release 1 μmol of reducing sugar per minute from a carboxymethyl cellulose solution with a concentration of 10 mg / ml is an activity unit (IU) , calculated as IU / ml.

[0036] Add 0.5ml of CMC substrate to each of the five test tubes, preheat together with the enzyme solution to be tested in a water bath at 50°C for 5 minutes; add 0.5ml of the enzyme solution to be tested to each of the first, second, third and fourth test tubes, and time, ℃ water bath for 15 minutes; after the reaction is completed, add 1.5ml of DNS reagent to each of the five test tubes, and add 0.5ml of the enzyme solution to be tested to the fifth test tube; React in the bath for 5 minutes; quickly cool to room temperature, and dilute to 5ml with water. Measure the absorbance of the test solutions in the first, second, third and ...

Embodiment 2

[0064] Embodiment 2: the acid cellulase of low temperature biological polishing in the biological ferment washing of denim washing

[0065] Fabric: 12 pieces of 40s / 1 pure indigo knitted jeans, 12 pieces of 10*10 woven blue and black jeans, weighing about 10kg;

[0066] Washing equipment: industrial washing machine

[0067] Process: Put the above 24 pairs of jeans into the industrial washing machine, add water to 100L, the total hardness of the water is 100-200ppm, heat the water to 35°C by steam heating, adjust the pH value of the solution to about 5.0, and the rotation speed is 35 rpm , Turn on and turn the industrial washing machine, add low-temperature desizing enzyme, low-temperature acid cellulose enzyme solution and anti-staining powder while turning, keep warm and turn for 40 minutes, drain the solution, and wash with water twice.

[0068] The ratio of this low-temperature acid cellulase solution is: acid cellulase: 550 parts; pectinase: 80 parts; alcohol ether: 150 p...

Embodiment 3

[0071] A low-temperature biopolishing acid cellulase preparation, comprising the following components by weight:

[0072] Enzyme activity is 10000IU / ml acid cellulase: 500 parts;

[0073] Enzyme activity is 5000IU / ml pectinase: 100 parts;

[0074] Alcohol ether: 50 parts;

[0075] Sodium chloride: 50 parts;

[0076] Calcium chloride: 50 parts;

[0077] Casson: 3 parts;

[0078] Deionized water: 300 parts.

[0079] The structure of the alcohol ether is: EO-PO-EO, wherein EO is ethylene oxide, PO is propylene oxide, the number of EO is 3-15, and the number of carbon chains is C 12 -C 18 .

[0080] The acid cellulase preparation of above-mentioned low-temperature biological polishing is prepared by the following method:

[0081] 1) Pour the weighed deionized water, Cathone, calcium chloride and sodium chloride into the mixing tank at a speed of 30r / min, and stir at room temperature until completely dissolved;

[0082] 2) Pour the weighed alcohol ether into the above solu...

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Abstract

The invention relates to an acid cellulase preparation for low-temperature biological polishing and a preparation method thereof. The acid cellulase preparation for the low-temperature biological polishing is prepared from the following components in parts by weight: 500 to 650 parts of acid cellulase, 30 to 100 parts of pectinase, 50 to 150 parts of alcohol ether, 50 to 100 parts of sodium chloride, 0 to 50 parts of calcium chloride, 0 to 3 parts of kathon and 50 to 300 parts of deionized water. The preparation method comprises the following steps: (1) pouring the weighed deionized water, kathon, calcium chloride and sodium chloride into a stirring tank, stirring at normal temperature until the kathon, the calcium chloride and the sodium chloride are dissolved completely, wherein the rotating speed is 30 to 50 r / min; (2) pouring the weighed alcohol ether into the solution, regulating the rotating speed to be 10 to 20 r / min, and stirring at the normal temperature until the alcohol ether is mixed uniformly; (3) pouring the weighed pectinase and acid cellulase into the solution, regulating the rotating speed to be 10 to 20 r / min, and stirring at the normal temperature; (4) after allcomponents are thrown into the stirring tank, stirring for 10 minutes, discharging 10 to 20 parts of the mixture from the bottom of the stirring tank, and pouring the 10 to 20 parts of the mixture into the stirring tank from the top of the stirring tank; (6) after the mixture is stirred for 5 minutes, discharging and filling while stirring. Compared with the prior art, the acid cellulase preparation has the advantages that the acid cellulase preparation can have a high-efficiency polishing effect and the like at the low temperature of 20 to 40 DEG C.

Description

technical field [0001] The invention relates to a fabric treatment agent, in particular to a low-temperature bio-polishing acid cellulase preparation and a preparation method thereof. Background technique [0002] Enzymes are biocatalysts that are synthesized in organisms under certain conditions according to the needs of life activities. Enzymes are involved in almost all chemical reactions in organisms and are the essential material basis for life activities. Due to the remarkable characteristics of strong specificity, high catalytic efficiency and mild reaction conditions, biological enzymes are widely used in many fields such as food, light industry, medicine, environmental protection, energy, etc., and the field of textile printing and dyeing is no exception. [0003] Cellulase is an enzyme preparation that is widely used in the field of textile printing and dyeing. Cellulase is mainly divided into acid cellulase and neutral cellulase. Cellulase polishing is mainly a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00D06M11/13D06M11/155D06M15/53
Inventor 吴浩吴齐金蒋秀玲郑慧春孙龙龙
Owner FIBER CHEM BIOLOGICAL CHEM
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