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Glioma prognostic marker circ15:101235082|101235577 and its application

A technology with good prognosis for glioma, applied in the fields of biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc.

Active Publication Date: 2020-06-30
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker circ15:101235082|101235577 and its application
  • Glioma prognostic marker circ15:101235082|101235577 and its application
  • Glioma prognostic marker circ15:101235082|101235577 and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of reagents for detecting the expression of circRNA circ15:101235082|101235577 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0019] 1. RNA stabilization solution 50ml

[0020] 2. Isopropanol 100ml

[0021] 3. Chloroform 100ml

[0022] 4. Trizol 50ml

[0023] 5. Enzyme-free water 10ml

[0024] 6.1 μM random reverse transcription primer 50 μl

[0025] 7.5× reverse transcription buffer 200ml

[0026] 8. 10mM base triphosphate deoxynucleotides 100μl

[0027] 9.40U / μl RNase inhibitor 500μl

[0028] 10.200U / μl MMLV reverse transcriptase 50μl

[0029] 11.Premix Ex Taq 50μl

[0030] 12.10μM circRNA circ15:101235082|101235577 real-time fluorescence quantitative PCR specific primer 30μl

[0031] circRNA circ15:101235082|101235577 Forward primer: 5'-ACCACGGAAGAAATGGGA-3',

[0032] circRNA circ15: 101235082|101235577 Reverse primer: ;5'-CAGATGTGTCAGAACCCTCA-3'

[0033] 13.10 μM GAPDH specific primer 30 μl

[003...

Embodiment 2

[0036] Example 2 Detection of the expression level of circRNA circ15:101235082|101235577 in brain tissue samples

[0037] 1. Collect the glioma or normal brain tissue to be tested, put it into a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0038] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add 200μl of chloroform to the tube, shake it by hand for 15-30s, place on ice for 5min, centrifuge at 12000rpm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add 0.5ml of pre-cooled isopropanol to mix well, and place ...

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Abstract

The invention belongs to the biotechnical field, and discloses a glioma prognostic marker circ15:101235082|101235577 and applications, namely a reagent for detecting circRNA circ15:101235082|101235577coming from brain tissues; and the glioma prognostic marker is used for preparing a prognostic preparation for patients with glioma. Study proves that patients with glioma having higher expression quantity of circRNA circ15:101235082|101235577 have higher postoperative survival rate. The prognostic prediction on the patients with glioma can be made by detecting the expression level of circRNA circ15:101235082|101235577 in glioma tissues of patients with glioma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker for glioma prognosis, the application of a reagent for detecting the marker for preparing a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
Inventor 武明花周和诚李沛瑶王维蒋健晖付海娟冯剑波
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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