Method and device for cell programmed layout seeding for cell impedance detection research

Abstract

The invention discloses a cell program control layout inoculation method and device for cell resistance detection study. An interdigital electrode is mounted at the internal bottom of a cell culture cavity and comprises a working electrode leading-out gold film, a counter electrode leading-out gold film and N strip gold electrodes, the working electrode leading-out gold film is parallel to the counter electrode leading-out gold film, the strip gold electrodes are perpendicular to the working electrode leading-out gold film and the counter electrode leading-out gold film, M miniature holes areequidistantly formed in a center line between two ends of each strip gold electrode, and a micro-magnetic pole is arranged under each miniature hole to form a micro-magnetic pole array comprising M*Nmicro-magnetic poles. By the aid of energized micro-magnetic pole attraction and cell immunomagnetic bead technology, the positions of energized micro-magnetic poles in the micro-magnetic pole array is determined according to preliminary cell inoculation layout, the positions and the number of the energized micro-magnetic poles in the micro-magnetic pole array are automatically determined by meansof program control, so that inoculated cell layout is as same as an expected target, and cell program control layout inoculation and automatic inoculation are realized.

Description

technical field [0001] The invention belongs to the field of cell impedance detection, in particular to a cell program-controlled layout inoculation method and device. Background technique [0002] Cell impedance detection technology has been widely used in the research of cell biology. Impedance detection when cells are attached to interdigitated electrodes can effectively evaluate changes in cell activity, cell proliferation, cell migration speed, and cell resistance to viruses. ability etc. However, in the process of using interdigitated electrodes for cell impedance detection, the distribution of cells on the surface of the interdigitated electrode cell sensor (such as the uniformity of distribution, distribution position, and density of distribution, etc.) is closely related to the accuracy of cell impedance detection. . For the various cell layout inoculation methods, the current main researches are antibody inoculation method, template inoculation method, etc. Amon...

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Problems solved by technology

The template inoculation method needs to make the inoculation template in advance, the inoculation layout of the template cannot be changed, and the inoculation layout of the cells cannot be adjusted and changed dynamically again during the inoculation process, because the requirements for automatic inoculation cannot be met because of the need for manual laying and removal of the template

Therefore, in the process of cell impedance detection research, it is impossible to study the same batch of cells in different distribution layout states, prolonging the research cycle

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