Pichia kudriavzevii strain for preparing beta-phenethyl alcohol through biological transformation of L-phenylalanine, culture method and applications thereof
A technology of yeast strain and phenylethanol, which is applied in the field of microorganisms to achieve the effects of optimizing ingredients and culture conditions, increasing production, and high ethanol tolerance
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Example 1 Pichia pastoris Kudri Azwitz ( Pichia kudriavzevii ) Isolation of Y1511
[0048] After pulverizing Gujing Gong Daqu and mixing it evenly, weigh 1 g, add 9 mL of sterile water, oscillate fully and soak for 15 min to make a suspension. Under aseptic conditions, take 0.1 mL of the suspension and dilute it step by step with sterile water to 10 -3 、10 -4 、10 -5 、10 -6 . Take 0.1 mL of each gradient suspension and spread it on the YPD plate, make three parallels for each dilution gradient, and spread sequentially from the low dilution concentration to the high dilution concentration. Place the medium plate at 30 o C cultured in a constant temperature incubator for 2 days to observe the growth of colonies. Pick up a single colony that is spherical, protruding on the surface, milky white, and opaque on the plate, and inoculate it on the YPD plate for multiple times until the bacteria are in the same shape under the microscope. Inoculate a single colony in 50 ...
Embodiment 2
[0049] Example 2 Preservation of Pichia kudriavzevii Y1511
[0050] The Pichia pastoris Kudri Azwitz obtained above ( Pichia kudriavzevii ) Y1511 is saved by the following methods:
[0051] (1) Preservation on slant: Inoculate the purified strains on the YPD slant, place it in the incubator for 48-72 h, and then store it at 4 o C stored in the refrigerator.
[0052] (2) Storage in glycerol tubes: Take 6 mL of 20% glycerol on the purified strain plate, scrape off the colony and mix it with 20% glycerol, divide the mixture into 1.5 mL sterile PE tubes, store at -80 o Save under C.
Embodiment 3
[0053] Example 3 Identification of Pichia kudriavzevii Y1511
[0054] The identification of the above mentioned Pichia kudriavzevii Y1511 includes the following steps:
[0055] Step 1: Morphological Characterization
[0056] In order to identify the yeast strains involved, the following morphological characteristics were observed:
[0057] (1) Colony morphology and cell observation in YPD medium: the pure culture of the strain obtained in Example 1 was inoculated on YPD medium, and its morphological characteristics were observed after 48 h ( figure 1 ), the results are shown in Table 1, and observed under an optical microscope after being magnified 400 times, the results are as follows figure 2 , presenting a long and narrow spindle, a small amount is oval, budding.
[0058] (2) Observation of colony morphology in WL medium: inoculate the screened strains on YPD for activation, culture for 24-48 h and then inoculate into WL medium, 30 o C cultured and observed after 5 da...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com