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Compound, conjugate, kit, and application of kit in detection of estradiol

A technology of compounds and conjugates, which is applied in the field of analysis, can solve problems such as estradiol derivatives to be developed, and achieve the effect of being easy to popularize, apply and obtain

Active Publication Date: 2018-02-02
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, currently applicable estradiol derivatives are still to be developed

Method used

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  • Compound, conjugate, kit, and application of kit in detection of estradiol
  • Compound, conjugate, kit, and application of kit in detection of estradiol
  • Compound, conjugate, kit, and application of kit in detection of estradiol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] In this example, the compound represented by formula (2) was synthesized according to the following process:

[0059]

[0060] The synthetic route is as follows:

[0061]

[0062] The specific synthesis steps are as follows:

[0063] 1. Synthesis of compound 2:

[0064] Charge 2.72g (0.01mol) 17β-estradiol (Compound 1) into a 100mL dry and cooled reaction flask, add 40mL dimethylformamide (DMF), stir to dissolve, and then add 3.14g (0.03mol) anhydrous potassium carbonate Under stirring, slowly add 2.17g (0.012mol) methyl 4-bromobutyrate dropwise. After the addition is complete, react for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, the organic layers were combined, and then washed three times with saturated brine, then dried over anhydrous sodium sulfate, and concentrated to obtain 4.7 g of crude compound 2.

[0065] MS: positive ion peak: 373.6, 395.6 (+Na peak); negative ion peak: 371.5.

[0066] 2. Synthesis of compound ...

Embodiment 2

[0070] In this example, the compound represented by formula (3) was synthesized according to the following process:

[0071]

[0072] The synthetic route is as follows:

[0073]

[0074] The specific synthesis steps are as follows:

[0075] 1. Synthesis of compound 5:

[0076] Charge 2.70g (0.01mol) estrone (compound 4) in a 100mL dry and cooled reaction flask, add 40mL DMF, stir to dissolve, then add 3.14g (0.03mol) anhydrous potassium carbonate, and slowly add 2.17g( 0.012 mol) Methyl 4-bromobutyrate. After the addition is complete, react for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, and the organic layers were combined, washed three times with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 4.6 g of crude compound 5.

[0077] MS: positive ion peak: 371.5, 393.5 (+Na peak); negative ion peak: 369.5.

[0078] 2. Synthesis of compound of formula (3):

[0079] Charge 4.6 g of the crude compound 5 ...

Embodiment 3

[0082] In this example, the compound represented by formula (4) was synthesized according to the following process:

[0083]

[0084] synthetic route

[0085]

[0086] The specific synthesis steps are as follows:

[0087] 1. Synthesis of compound 8:

[0088] Charge 2.88g (0.01mol) estriol (compound 7) in a 100mL dry and cooled reaction flask, add 40mL DMF, stir to dissolve, add 3.14g (0.03mol) anhydrous potassium carbonate, and slowly add 2.17g (0.012 mol) Methyl 4-bromobutyrate. After the addition is complete, react for 4 hours. The reaction solution was poured into ice water, extracted three times with ethyl acetate, and the organic layers were combined, then washed three times with saturated brine, dried over anhydrous sodium sulfate, and concentrated to obtain 4.9 g of crude compound 8.

[0089] MS: positive ion peak: 389.7, 411.7 (+Na peak); negative ion peak: 387.8.

[0090] 2. Synthesis of compound of formula (4):

[0091] Feed 4.9 g of the crude compound 8 obtained in the previ...

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Abstract

The invention discloses a compound, a conjugate, a kit for detecting estradiol, and an application of the kit for detecting the estradiol in detection of the estradiol. The compound has a structure shown as a formula (1), wherein the L represents a connecting arm, the R<1>, R<2>, R<3> and R<4> are respectively independently a hydrogen group, a hydroxyl group, an alkyl of C<1-3>, an alkoxy of C<1-3>, an alkenyl of C<2-3> or an alkynyl of C<2-3>. The compound disclosed by the invention can be used for accurately detecting estradiol.

Description

Technical field [0001] The invention relates to the field of analysis. Specifically, the present invention relates to compounds, conjugates, kits and their use in detecting estradiol. More specifically, the present invention relates to the use of compounds, conjugates, kits for detecting estradiol, and kits for detecting estradiol in detecting estradiol. Background technique [0002] Estrol refers to a substance with the following nucleus structural units, the common ones are estrone, estradiol and estriol. [0003] [0004] At present, the principle of using immunoassays to detect estradiol is: since there is no functional group on estradiol that can be directly connected to the labeling molecule, it needs to be chemically synthesized to introduce a structure that can be directly connected to the labeling molecule (called a linker, and the terminal functional group can be Is carboxyl, amino, etc.) to obtain estradiol derivatives. Then, the linking arm of the estradiol derivativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J1/00C12N9/08C12N9/16C12N9/48G01N33/74G01N33/68G01N33/535C12Q1/42C12Q1/37C12Q1/28
CPCC07J1/0059C07J1/0062C07J1/007C12N9/0065C12N9/16C12N9/48C12Q1/28C12Q1/37C12Q1/42C12Y111/01007C12Y301/03001G01N33/535G01N33/6854G01N33/743
Inventor 唐涛张裕平何飞
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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