Method for determining form of trace selenium, and applications of method in detection of selenium-rich feed

A technology of selenium element and form, which is applied in the field of feed quality inspection, can solve the problems of inability to accurately measure and analyze the form of selenium in selenium-enriched feed, and achieve the effects of fast and safe selection and application, mild enzymatic hydrolysis system conditions, and accelerated enzymatic reactions

Pending Publication Date: 2018-02-23
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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Problems solved by technology

[0007] For this reason, the technical problem to be solved by the present invention is to provide a method for measuring the form of selenium in selenium-enriched feed, to solve the problem in the prior art that the form of selenium in selenium-enriched feed cannot be accurately measured and analyzed

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  • Method for determining form of trace selenium, and applications of method in detection of selenium-rich feed
  • Method for determining form of trace selenium, and applications of method in detection of selenium-rich feed
  • Method for determining form of trace selenium, and applications of method in detection of selenium-rich feed

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Embodiment 2

[0080] Embodiment 2 Determination of selenium form in a well-known brand selenium-enriched feed additive

[0081] The method for measuring the form of trace selenium in the selenium-enriched feed described in this embodiment specifically includes the following steps:

[0082] (1) Enzymatic extraction: Weigh 20-50 mg of selenium-enriched feed sample into a 50 mL centrifuge tube, add 10 mg of protease XIV and 25 mL of Tris buffer, vortex and mix for 1 min; place the ultrasonic probe in the sample solution, set the output The power is 50%, and the ultrasonic probe assisted enzymatic hydrolysis at room temperature for 60s; the sample solution after enzymatic hydrolysis treatment was taken out and centrifuged at 10000r / min for 5min at 4°C, and the supernatant was passed through a 0.2μm aqueous phase filter membrane to prepare the sample to be tested solution;

[0083] (2) Determination of selenium speciation: using high performance liquid chromatography-hydride generation atomic f...

Embodiment 3

[0094] Example 3 Determination of selenium speciation of a certain brand of selenium-enriched feed additive

[0095] The method for measuring the form of trace selenium in the selenium-enriched feed described in this embodiment specifically includes the following steps:

[0096] (1) Enzymatic extraction: Weigh 20-50 mg of selenium-enriched feed sample into a 50 mL centrifuge tube, add 10 mg of protease XIV and 25 mL of Tris buffer, and vortex for 1 min. The ultrasonic probe was placed in the sample solution, the output power was set to 50%, and the ultrasonic probe assisted enzymatic hydrolysis at room temperature for 60 s. Take out the sample solution after enzymatic hydrolysis treatment and centrifuge at 10000r / min for 5min at 4°C, and pass the supernatant through a 0.2μm aqueous phase filter membrane to prepare the sample solution to be tested;

[0097] (2) Determination of selenium speciation: using high performance liquid chromatography-hydride generation atomic fluoresc...

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Abstract

The invention belongs to the technical field of feed quality detection, and particularly to a method for determining the selenium form in a selenium-rich feed, more particularly to a method for determining the selenium form in a selenium-rich feed by combining an ultrasonic probe-assisted enzymolysis technology and high-performance liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS). According to the present invention, with the method, the content of the extracted selenium can be accurately determined, and the contents of the five forms of the selenium substances commonly contained in the selenium-rich feed can be effectively separated, such that the selenium form in the selenium-rich feed can be accurately analyzed, and the selenium-rich feed product can be rapidly and safely selected and used.

Description

technical field [0001] The invention belongs to the technical field of feed quality detection, in particular to a method for determining the form of selenium in selenium-enriched feed, and more specifically to a method based on ultrasonic probe-assisted enzymolysis technology combined with high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy (HPLC). -HG-AFS) method for determining the form of selenium in selenium-enriched feed. Background technique [0002] Selenium (Selenium, Se) is one of the 14 trace elements necessary for humans and animals, and is an essential component of selenocystine, selenomethionine and selenium-containing enzymes (such as glutathione peroxidase). And play an important role in the body's anti-cancer, anti-oxidation and other aspects. According to research, selenium deficiency in animals will directly affect the physiological functions of important organs such as bones, liver, and thyroid gland, and can lead to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 肖志明樊霞刘成新贾铮王石刘晓露李阳田静
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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