Method for rapidly identifying cassava autotetraploid

An autotetraploid and identification method technology, applied in the field of autotetraploid identification, can solve the problems of expensive experimental instruments, toxic reagents, harmful to the body, etc., and achieve rapid identification, easy operation, and high accuracy

Inactive Publication Date: 2018-03-02
广西壮族自治区农业科学院经济作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The advantages are high identification efficiency and accurate results; the disadvantages are that the experimental equipment is relatively expensive, the identification cost is too high, and th

Method used

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  • Method for rapidly identifying cassava autotetraploid
  • Method for rapidly identifying cassava autotetraploid
  • Method for rapidly identifying cassava autotetraploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Material collection: When the cassava plant grows to 0.8 meters, the sixth expanded leaf of cassava variety Xinxuan 048 with unknown ploidy is taken as the material for identifying the autotetraploid of the plant.

[0033] (2) Film production: After wiping off the dust on the leaves, place them on the operating table. Use a scalpel to draw a cross on the lower epidermis of the leaf avoiding the veins, lift up the corner of the cross with tweezers, slowly tear off the lower epidermis of the leaf, immerse the lower epidermis of the leaf in Carnot’s fixative solution for 2 seconds, then take it out quickly, and dry the lower epidermis with filter paper Finally, place the lower epidermis on a clean glass slide, and stain with a drop of iodine-potassium iodide staining solution for 60 seconds. Cover with a coverslip and blot excess liquid with filter paper.

[0034] (3) Counting: Place the prepared sheet under a 40 times objective lens of an ordinary optical microscope ...

Embodiment 2

[0037] (1) Material collection: when the cassava plant grows to 0.6 meters, the seventh unfolded leaf of cassava variety Huanan 205 with unknown ploidy is taken as the material for identifying the autotetraploid of the plant.

[0038] (2) Film production: After wiping off the dust on the leaves, place them on the operating table. Use a scalpel to draw a cross on the lower epidermis of the leaf avoiding the veins, lift up the corner of the cross with tweezers, slowly tear off the lower epidermis of the leaf, immerse the lower epidermis of the leaf in Carnot’s fixative solution for 2 seconds, then take it out quickly, and dry the lower epidermis with filter paper Afterwards, place the lower epidermis on a clean glass slide, and stain with a drop of iodine-potassium iodide staining solution for 120s. Cover with a coverslip and blot excess liquid with filter paper.

[0039] (3) Counting: Place the prepared sheet under a 40 times objective lens of an ordinary optical microscope fo...

Embodiment 3

[0042] (1) Material collection: When the cassava plant grows to 1.0 m, the fifth expanded leaf of cassava variety Nanzhi 199 with unknown ploidy is taken as the material for identifying the autotetraploid of the plant.

[0043](2) Film production: After wiping off the dust on the leaves, place them on the operating table. Use a scalpel to draw a cross on the lower epidermis of the leaf avoiding the veins, lift up the corner of the cross with tweezers, slowly tear off the lower epidermis of the leaf, immerse the lower epidermis of the leaf in Carnot’s fixative solution for 2 seconds, then take it out quickly, and dry the lower epidermis with filter paper Afterwards, place the lower epidermis on a clean glass slide, and stain with a drop of iodine-potassium iodide staining solution for 30 seconds. Cover with a coverslip and blot excess liquid with filter paper.

[0044] (3) Counting: Place the prepared sheet under a 40 times objective lens of an ordinary optical microscope for ...

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Abstract

The invention relates to a method for rapidly identifying cassava autotetraploid, and belongs to the technical field of autotetraploid identification. The method for rapidly identifying the cassava autotetraploid comprises the following steps: 1) immersing a cassava leaf in a Carnot fixer to obtain a fixed cassava leaf; 2) taking the lower epidermis of the fixed cassava leaf obtained in step 1), and staining the lower epidermis in an iodine-potassium iodide staining solution to obtain a stained lower epidermis; and 3) microscopically observing the stained lower epidermis obtained in step 2), counting the number of chloroplasts in the guard cells, determining the cassava as an autoteraploid if the number of chloroplasts in more than 90% of the guard cells in the visual field is 7 or more, and determining the cassava as a diploid if the number of chloroplasts in more than 90% of the guard cells in the visual field is less than 7. The identification method has the advantages of low cost,high speed, high accuracy, and realization of efficient and rapid detection of the cassava autotetraploid.

Description

technical field [0001] The invention relates to the technical field of autotetraploid identification, in particular to a rapid identification method for cassava autotetraploid. Background technique [0002] Cassava (Manihotesculenta Crantz) is a plant of the Euphorbiaceae (Euphorbiaceae) cassava genus (Manihot). It is drought-resistant and barren-resistant. It is widely planted in more than 100 countries and regions such as Africa, America and Asia. It is known as the "king of starch" and is the world's Nearly 600 million people depend on food for survival. Cassava has a wide range of uses. In addition to being used as food, it is also an important industrial raw material, such as cassava root starch, dry starch that can be made into modified starch, alcohol and sorbitol, etc., and these products are also important raw materials for food, medicine, textile, paper and other industries. . [0003] Cassava is diploid, the number of chromosomes is 2n=36, and it is a crop for a...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N15/14G01N21/84
CPCG01N1/30G01N15/1475G01N21/84
Inventor 严华兵周慧文曹升谢向誉尚小红肖亮曾文丹陆柳英
Owner 广西壮族自治区农业科学院经济作物研究所
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