In-situ remediation method of black odor sediment in contaminated static water body
A black and odorous bottom sludge and in-situ repair technology, applied in the direction of biological sludge treatment, can solve the problems of aggravated water pollutants, inability to function for a long time, unstable growth environment and action time of biological agents, etc., and achieve the goal of reducing the impact Effect
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Embodiment 1
[0037] This embodiment provides an in-situ repair method for black and odorous bottom mud in polluted static water bodies, comprising the following steps:
[0038] Step 101: Prepare compound microbial preparation:
[0039] The composition of the composite microbial preparation is (mass ratio) Bacillus fermentation broth: fatty alcohol polyoxyethylene: Tween 80: urea: diammonium hydrogen phosphate: EDTA: distilled water=44:5:0.1:0.1:0.1:0.0001:50.7, The composition ratio of the bacillus fermented liquid is (number of bacteria ratio) Bacillus subtilis: Bacillus amyloliquefaciens: Bacillus pumilus: Bacillus licheniformis: Bacillus megaterium=10×10 7 CFU / ml: 6×10 7 CFU / ml: 3×10 7 CFU / ml: 1.5×10 7 CFU / ml: 1.5×10 7 CFU / ml. The specific preparation method is as follows:
[0040] (1) The preparation of seed solution comprises the following steps:
[0041] Activation of slant strains: the slant test tubes of Bacillus mohaiwei and Bacillus pumilus were cultured in a constant temp...
Embodiment 2
[0062] This embodiment provides an in-situ repair method for black and odorous bottom mud in polluted static water bodies, comprising the following steps:
[0063] Step 201: Prepare compound microbial preparation:
[0064] The composition of the composite microbial preparation is (mass ratio) Bacillus fermentation broth: fatty alcohol polyoxyethylene: Tween 80: urea: diammonium hydrogen phosphate: EDTA: distilled water=60:1:0.3:0.3:0.3:0.0002:38, The composition ratio of the bacillus fermented liquid is (number of bacteria ratio) Bacillus subtilis: Bacillus amyloliquefaciens: Bacillus pumilus: Bacillus licheniformis: Bacillus megaterium=10×10 7 CFU / ml: 6×10 7 CFU / ml: 3×10 7 CFU / ml: 1.5×10 7 CFU / ml: 1.5×10 7 CFU / ml.
[0065] (1) The preparation of seed solution comprises the following steps:
[0066] Activation of slant strains: the slant test tubes of Bacillus mohaiwei and Bacillus pumilus were cultured in a constant temperature incubator at 32°C for 48 hours.
[0067] ...
Embodiment 3
[0087] This embodiment provides an in-situ repair method for black and odorous bottom mud in polluted static water bodies, comprising the following steps:
[0088] Step 301: Prepare compound microbial preparation:
[0089] The composition of the composite microbial preparation is (mass ratio) Bacillus fermentation broth: fatty alcohol polyoxyethylene: Tween 80: urea: diammonium hydrogen phosphate: EDTA: distilled water=55:3:0.25:0.25:0.25:0.0002:41.25, The composition ratio of Bacillus fermented liquid is (bacteria number ratio) Bacillus subtilis: Bacillus amyloliquefaciens: Bacillus pumilus: Bacillus licheniformis: Bacillus megaterium=9×10 7 CFU / ml: 3×10 7 CFU / ml: 1.4×10 7 CFU / ml: 1.4×107CFU / ml: 1.4×107CFU / ml.
[0090] The specific steps of preparing the compound microbial preparation are the same as those in Example 1 and Example 2, the concentration of the fermentation broth is adjusted in proportion, and the insufficient part can be supplemented with distilled water.
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