Anti-ageing and whitening composition containing sweet osmanthus flower stem cell extract and preparation method thereof
A stem cell and anti-aging technology, applied in the direction of medical preparations containing active ingredients, skin care preparations, pharmaceutical formulas, etc., can solve the problems of not being able to reach the deep layer of the skin and unsatisfactory effects, and achieve high free radical scavenging rate and anti-aging Good aging effect, good whitening effect
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[0021] The preparation method of osmanthus fragrans stem cell extract of the present invention comprises the following steps:
[0022] 1) Isolation and cultivation of osmanthus fragrans cambium stem cells: After sterilizing the leaves of osmanthus osmanthus, under aseptic conditions, cut the sterilized leaves of osmanthus fragrans into 0.5cm*0.5cm size, inoculate them on WPM medium, and make the back of the leaves In contact with WPM medium, cultivated in the dark at 25°C; two weeks later, the cambium stem cells were transferred to the subculture medium for culture, and subcultured once every two weeks to obtain the cambium stem cells of Osmanthus fragrans;
[0023] 2) Collect the medium after culturing the cambium stem cells of Osmanthus osmanthus, filter through an ultrafiltration membrane, concentrate the filtrate, remove the phenol red in the medium, and keep the active ingredients in the medium; the lysates of the medium and the osmanthus cambium stem cells are frozen Dry...
Embodiment 1
[0025] An embodiment of the anti-aging and whitening composition of the present invention, the composition includes the following components by weight: 12 parts of sweet-scented osmanthus stem cell extract, 6 parts of white lupine protein, 12 parts of bearberry leaf extract .
Embodiment 2
[0027] An embodiment of the anti-aging and whitening composition of the present invention, the composition includes the following components by weight: 10 parts of sweet-scented osmanthus stem cell extract, 10 parts of white lupine protein, 12 parts of bearberry leaf extract .
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