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Method for tissue culture and quick propagation of brucea javanica

A technology of tissue culture and brucei, applied in the field of biological reproduction, can solve problems such as difficulties in introduction and domestication, endangered wild resources, destruction of ecological environment, etc., and achieves the effects of maintaining excellent characters, increasing reproduction coefficient, and improving safety.

Inactive Publication Date: 2018-03-30
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, wild resources are mainly used for medicinal purposes, and the blind collection of wild resources will inevitably cause damage to the ecological environment and the endangerment of wild resources, and the difference in the ecological environment makes introduction and domestication very difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Step 1, soaking the sterilized explants in a 1% soluble polyvinylpyrrolidone solution for 5 minutes to obtain sterile explants;

[0042] Step 2, utilize the induction medium that comprises 6-BA 1.0mg, 2,4-D 1.0mg, NAA 1.5mg, agar 4.5g, sucrose 25g and distilled water 0.9 liters to induce the described sterile explant and at a temperature of 24 Cultivate for 7 days under dark culture conditions at ℃, then culture for 21 days under the conditions of light intensity of 1500 lux and time of 10 hours / day to obtain callus;

[0043] Step 3. Cultivate the callus with a differentiation medium comprising 6-BA2.0mg, agar 4.5g, sucrose 25g and distilled water 0.9 liters at a temperature of 26°C, a light intensity of 1500 lux, and a light time of 12 hours / day , cultivated for 21 days to obtain the clustered buds.

[0044] Step 4. Cultivate in a proliferation medium comprising 0.3 mg of TDZ, 0.5 mg of 6-BA, 4.5 g of agar, 25 g of sucrose and 0.9 liters of distilled water and culture...

Embodiment 2

[0049] Step 1, soaking the sterilized explant in a soluble polyvinylpyrrolidone solution with a mass concentration of 1% after being sterilized by a filter membrane for 8 minutes to obtain a sterile explant;

[0050] Step 2, utilize the induction medium that comprises 6-BA 1.3mg, 2,4-D 2.0mg, NAA2.0mg, agar 4.5g, sucrose 25g and distilled water 0.92 liters to induce the described sterile explants and at a temperature of 25 Cultivate for 7 days under dark culture conditions at ℃, and then culture for 21 days under the conditions of light intensity of 1500 lux and time of 11 hours / day to obtain callus;

[0051] Step 3. Cultivate the callus with a differentiation medium comprising 1.0 mg of 6-BA, 4.5 g of agar, 25 g of sucrose and 0.92 liters of distilled water and culture the callus at a temperature of 25° C., a light intensity of 1500 lux, and a light time of 11 hours / day Under the condition of culturing for 21 days, the clustered buds were obtained.

[0052] Step 4, using the...

Embodiment 3

[0057] Step 1, soaking the sterilized explant in a 1% soluble polyvinylpyrrolidone solution for 10 minutes to obtain a sterile explant;

[0058] Step 2, utilize the induction medium that comprises 6-BA 1.5mg, 2,4-D 2.5mg, NAA2.5mg, agar 4.5g, sucrose 25g and distilled water 0.95 liters to induce the described sterile explants and at a temperature of 26 Cultivate for 7 days under dark culture conditions at ℃, then culture for 21 days under the conditions of 1500 lux light intensity and 12 hours / day to obtain callus tissue;

[0059] Step 3. Cultivate the callus with a differentiation medium comprising 1.0 mg of 6-BA, 4.5 g of agar, 25 g of sucrose and 0.95 liters of distilled water and culture the callus at a temperature of 25° C., a light intensity of 1500 lux, and a light time of 11 hours / day Under the condition of culturing for 21 days, the clustered buds were obtained.

[0060] Step 4. Cultivate in a proliferation medium containing TDZ 0.5mg, 6-BA 1.5mg, agar 4.5g, sucrose ...

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PUM

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Abstract

The invention discloses a method for tissue culture and quick propagation of brucea javanica. The method comprises the following steps of step 1, manufacturing a sterile explant, wherein the explant is a tender leaf of brucea javanica; step 2, utilizing an induction culture medium to induce the sterile explant, so as to obtain callus; step 3, utilizing a differentiation culture medium to culture the healing tissue, so as to obtain multiple shoots; step 4, utilizing a proliferation culture medium to culture the multiple shoots, so as to obtain multiple shoots of a test-tube plantlet; step 5, using a strong seedling culture medium to culture the multiple shoots of the test-tube plantlet, so as to obtain a strong test-tube plantlet; step 6, utilizing a rooting culture medium to culture the strong test-tube plantlet, so as to obtain a rooting test tube seedling; 7, transplanting the rooting test tube plantlet into a matrix to culture. The method has the advantages that the wild resources can be more effectively utilized, the collection amount of branches is reduced, and the propagation coefficient is quickly increased.

Description

technical field [0001] The invention relates to the technical field of biological reproduction, in particular to a method for fast propagation of Brucea javanica tissue culture. Background technique [0002] Brucea javanica, also known as old javanica, Brucea javanica, bitter hazelnut, etc., is an evergreen shrub or small tree of the genus Brucea javanica in the family Bitterwoodaceae, distributed in Fujian, Taiwan, Guangdong, Guangxi, Hainan and Yunnan provinces and regions; Brucea javanica has It has many application values, especially in medical applications. It has the effects of clearing away heat and dampness, detoxification, killing insects, and corrosion. Meanwhile, Brucea javanica has pharmacological effects such as anti-tumor, anti-digestive ulcer, lowering blood fat, antibacterial and anti-inflammatory. At present, domestic and foreign research on Brucea javanica mainly focuses on component analysis, pharmacological effects, tissue culture and other aspects, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 韦树根潘丽梅付金娥白隆华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS