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Liquid for preserving red cells of ursid and application of liquid for preserving red cells of ursid

A technology of red blood cell and preservation solution, which is applied in the field of red blood cell preservation solution of bears, can solve the problems of being unsuitable for giant pandas, poor preservation effect of giant pandas, poor tolerance of human red blood cell preservation solution, etc.

Active Publication Date: 2018-04-06
SHANGHAI BLOOD CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preservation solutions used in the study of human red blood cell preservation include ACD preservation solution and CPD-A preservation solution. Not suitable for giant panda red blood cells
The reason may be speculated that due to the fragility of giant panda erythrocytes and the Na in plasma + The ion content is different from that of humans, and giant panda red blood cells are less tolerant to human red blood cell preservation solutions
Therefore aiming at the blood physiological characteristics of bears, it is necessary to develop a kind of bears erythrocyte preservation solution, and about the bears erythrocytes preservation solution of the present invention, there is no report yet

Method used

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  • Liquid for preserving red cells of ursid and application of liquid for preserving red cells of ursid
  • Liquid for preserving red cells of ursid and application of liquid for preserving red cells of ursid
  • Liquid for preserving red cells of ursid and application of liquid for preserving red cells of ursid

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Preservation Solution Preparation and Giant Panda Cell State Evaluation Experiment

[0030] Plasma osmotic pressure is the internal environment for the survival of red blood cells. Under normal circumstances, red blood cells and plasma are in a state of basic osmotic pressure equilibrium. When the plasma osmotic pressure changes, it will cause the living environment of blood cells to deteriorate, function limitation and even death. In order to reasonably configure the red blood cell preservation solution of bears, we firstly measured the osmotic pressure of giant panda plasma.

[0031] Since this experiment is aimed at the research on the physiological fragility of red blood cells of healthy giant pandas, and the blood samples of giant pandas are limited, a larger osmotic fragility gradient was selected in the meaningless range of reference to human dogs. In the meaningful fragility range of humans and dogs, We halved the gradient, so a saline gradient of 0....

Embodiment 2

[0046] Example 2 Giant Panda Red Blood Cell Preservation Experiment

[0047] Preparation of Giant Panda Red Blood Cell Preservation Solution:

[0048] 2× mother liquor formula

[0049]

[0050] In this experiment, the study on the fragility of giant panda red blood cells found that giant panda red blood cells are more sensitive to osmotic pressure than human red blood cells. For red blood cell preservation solutions with different pressures, adjust the osmotic pressure to 235 (1:0.85 / H2O: mother solution), 245 (1:0.92), 255 (1:1), and 265 (1:1.1). Labeled as B1, B2, B3, B4 four preservation solutions.

[0051] Observation on the appearance and cell morphology of giant panda red blood cells in vitro maintenance liquid for 30 days

[0052] From the comparison of fresh and aged giant panda red blood cells, we can find that the status of giant panda red blood cells can be judged by using a 100× optical microscope. Observation, it was found that the external shape of the cel...

Embodiment 3

[0055] Example 3 Detection of free hemoglobin in giant panda red blood cell preservation solution for 30 days

[0056] Due to the appearance, we have found that giant panda red blood cell preservation solution B3 has the best preservation effect on giant panda red blood cells. In order to verify this conclusion, we analyzed the free hemoglobin content in the maintenance supernatant, and the results are as follows Figure 6 As shown, it was found that at 10 days, 20 days, and 30 days, there were extremely significant differences between the B3 maintenance solution maintenance group and other groups (P<0.01). It further illustrates that the giant panda red blood cell preservation solution B3 is the best solution among the four groups.

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Abstract

The invention relates to a liquid for preserving red cells of a ursid. The liquid for preserving the red cells of the ursid comprises NaCl, NaH2PO4, ATP, glucose, mannitol and glutathione. According to the blood physiological characteristics of pandas, four liquids for preserving the red cells are prepared, and tests of free hemoglobins in supernatant of the liquids for preserving the red cells atdifferent time points indicate that: the B3 liquid has significant differences from other liquids on the tenth day, the B2 and B3 liquids have significant differences from the other two liquids on the 20th day, and the B2, B3 liquids have significant differences from the other two liquids on the 30th day. The results show that the B3 liquid is the best system for preserving the red cells; the preservation effect of the B3 liquid is verified by using ATP tests and 2,3-DPG tests, and results show that: the content of ATP and 2,3-DPG in the B3 liquid is significantly different from that in the B1 liquid, and it is indicated that the B3 liquid has a better effect on the preservation of the red cells of the pandas. The liquid for preserving the red cells can preserve the red cells of the ursideffectively for 30 days, and can be applied to clinical red cell transfusion of the ursid.

Description

technical field [0001] The invention relates to the technical field of blood preservation, and specifically discloses a bear red blood cell preservation solution and an application thereof. Background technique [0002] Panda blood transfusion is an important technical method urgently needed in giant panda veterinary clinics. Due to the lack of corresponding research, the blood components of giant pandas and their alternative blood sources, Asiatic black bears, cannot be stored in vitro for a long time, which hinders the use of red blood cell transfusions in giant pandas in the wild. Application in panda disease treatment and field rescue. How to preserve giant panda red blood cells for a relatively long period of time, so that giant pandas scattered around the world can use safe and high-quality red blood cell transfusion therapy when needed is an urgent problem to be solved. [0003] The lifespan of human red blood cells is about 120 days. The membrane fragility of damage...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 赵俸涌朱自严李德生邓林华钱旻李勤张嘉敏叶璐夷
Owner SHANGHAI BLOOD CENT
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