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Bioactive polypeptide qpevmgvskvkeamapkqkempfpky and its preparation and application

A bioactive polypeptide and drug technology, applied in the field of protein, can solve problems such as human body risks, achieve the effects of enhancing functions, scavenging free radicals, and reducing body aging

Active Publication Date: 2020-05-12
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • Bioactive polypeptide qpevmgvskvkeamapkqkempfpky and its preparation and application
  • Bioactive polypeptide qpevmgvskvkeamapkqkempfpky and its preparation and application
  • Bioactive polypeptide qpevmgvskvkeamapkqkempfpky and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The artificial synthesis of embodiment 1 active peptide QPEVMGVSKVKEAMAPKQKEMPFPKY

[0038] 1. Synthesis of Bioactive Peptides

[0039] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0040] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0041] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0042] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to test it (Two drops each of Test A and Test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

...

Embodiment 2

[0080] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0081] 1. Determination of in vitro antioxidant activity of bioactive peptide QPEVMGVSKVKEAMAPKQKEMPFPKY by [DPPH·] method

[0082] 1. Experimental reagents and instruments:

[0083] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Milk-derived bioactive peptide QPEVMGVSKVKEAMAPKQKEMPFPKY.

[0084] Main instruments: Sunrise microplate reader, product of Tecan Company of Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; analytical balance, product of Meitelei-tolido Company.

[0085] 2. Experimental method:

[0086] (1) 1mmol / L [DPPH·]methanol solution

[0087] Weigh 0.349 mg [DPPH·] with an analytical balance and dissolve it in 1 mL of methanol solution to prepare a 1 mmol / L [DPPH·] methanol solution, store it in...

Embodiment 3

[0120] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0121] 1. Experiment on the effect of biologically active polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY on the exercise ability of Caenorhabditis elegans

[0122] 1. Experimental reagents and instruments:

[0123] Reagents: Caenorhabditis elegans, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Escherichia coli strain E.coli OP50, Institute of Integrating Chinese and Western Medicine affiliated to Fudan University; Agar powder, Sinopharm Chemical Reagent Co., Ltd.; Yeast powder, Sinopharm Chemical Reagent Co., Ltd. Company; the milk-derived biologically active polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY obtained in Example 1.

[0124]Instruments and equipment: Likang RO15 pure water system, Likang Biomedical Technology Co., Ltd.; G136T Zealway intelligent high-temperature sterilization pot, Xiamen Zhiwei Instrument Technology Co., Ltd.; THZ-32 desktop constant temperature osci...

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Abstract

The invention relates to the field of proteins, in particular to bioactive polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY as well as preparation and application thereof. The amino acid sequence of the bioactive polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY is Gln-Pro-Glu-Val-Met-Gly-Val-Ser-Lys-ValLys-Glu-Ala-Met-Ala-Pro-Lys-Gln-Lys-GluMet-Pro-Phe-Pro-Lys-Tyr. In-vitro antioxidant experiments and in-vivo antiaging experiments prove that the polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY has better antioxidant bioactivity and antiaging activity; on one hand, the polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY has better antioxidant activity and can scavenge free radicals in the organism and improve quality of life; on the other hand, the activity of in-vivo anti-peroxidase system can be improved, the function of the organism for resisting exogenous stimulation is enhanced, the probability of aging, senility and illness of the organism is reduced, and the bioactive polypeptide has great significance in development of food, health care products and drugs with antioxidant and antiaging functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide QPEVMGVSKVKEAMAPKQKEMPFPKY and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soyb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A23L33/18A61K38/17A61K8/64A61P39/06A61Q19/08
CPCA23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08C07K14/4732A23V2250/55A23V2200/302
Inventor 张少辉张伯宇李阜烁林学海李云飞陈静范梦珠李婉如
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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