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A kind of breeding method of comprehensive hybrid of rapeseed

A breeding method and hybrid technology, which are applied in the field of rapeseed breeding, can solve the problems of obvious heterosis, low seed production yield, and high seed production cost, and achieve the effects of simple breeding procedures and methods, low seed production cost, and short breeding cycle.

Inactive Publication Date: 2020-07-28
QINGHAI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above-mentioned problems, the object of the present invention is to overcome the problems of low seed production per unit area, high seed production cost, and high seed price in the traditional rapeseed hybrid cultivation and production process. It is a method for utilizing heterosis, providing a method for cultivating comprehensive hybrids of rapeseed that does not require excision of the male parent, has high seed production per unit area, low cost of seed production, and obvious heterosis

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  • A kind of breeding method of comprehensive hybrid of rapeseed

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Embodiment 1

[0049]A method for cultivating comprehensive hybrids of rapeseed, comprising the following steps:

[0050] (1) CMS selection: The Polyma cytoplasmic male sterile line 025A (very early-maturing Brassica napus) with a petal opening angle of 65° and complete fertility was selected as the female parent of the comprehensive hybrid.

[0051] The opening angle of the petals of the sterile line is 65°, and the nectaries are tightly wrapped, which is beneficial for bees to pollinate, thereby ensuring the seed setting rate of the female parent.

[0052] (2) Restoration system selection:

[0053] Using the early-maturing Brassica napus line 249R (including the restorer gene) to cross with the extra-early-maturing Brassica napus self-incompatibility germplasm account 28, using the pedigree method, in the F2-F4 generation, the strains with good comprehensive traits were selected for self-bagging Artificially assisted pollination was carried out, and the self-compatibility index of each li...

Embodiment 2

[0058] A method for cultivating comprehensive hybrids of rapeseed, comprising the following steps:

[0059] (1) CMS selection: The Polyma cytoplasmic male sterile line 307A (very early-maturing Brassica napus) with a petal opening angle of 81°, tightly wrapped nectaries, and complete fertility was selected as the female parent of the comprehensive hybrid.

[0060] The opening angle of the petals of the sterile line is 81°, and the nectaries are tightly wrapped, so it is beneficial for bees to pollinate, thereby ensuring the seed setting rate of the female parent.

[0061] (2) Restoration system selection:

[0062] The early-maturing Brassica napus lines 039R and 249R (including restorer genes) were crossed with the extra-early-maturing Brassica napus self-incompatibility germplasm account 28, and the hybrid progenies of the two combinations were selected by pedigree method. F2~F4 In the next generation, the strains with good comprehensive traits were selected for self-bagging...

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Abstract

The invention belongs to the technical field of rape breeding, and in particular relates to a method for cultivating synthetical hybrid variety of rape by using a sterile line and a series of restorers. The method comprises the steps of selecting the sterile line: selecting the sterile line, which has a petal opening angle of greater than or equal to 65 degrees, has tightly wrapped nectaries and is thorough in fertility, as a female parent of the synthetical hybrid variety; selecting the restorers: selecting a plurality of restorers which have full recovery property, have self-compatible indexes of 1-5 and are high in combining ability, and mixing the selected restorers to form a hybrid restorer used as a male parent of the synthetical hybrid variety; preparing the synthetical hybrid variety: sowing the sterile line and the hybrid restorer in an isolated field in rows, carrying out free pollination, and carrying out mixed harvesting at the ripening stage to obtain the synthetical hybrid variety. The method has the advantages that after the series of restorers are selected and bred, the breeding procedures are simple, and the breeding efficiency is high; the hybrid variety can be also formed between the restorers, so that the good synthetical hybrid variety can be more easily screened out; the male parent does not need to be cut off, so that the variety production yield is increased, and the variety production cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for cultivating comprehensive hybrids of rapeseed by using a sterile line and a series of restorer lines. Background technique [0002] Utilization of heterosis is an effective way to increase the yield of rapeseed. The ways to utilize heterosis in rapeseed mainly include the following aspects: cytoplasmic male sterility, nuclear male sterility, chemical male sterility, ecological male sterility, self-incompatible lines and synthetic hybrids. In production practice, except for the utilization of comprehensive hybrids, the rest of the methods produce single-cross species, and need to cut male parents. Taking the currently widely used cytoplasmic male sterile three-line hybrid seed production as an example, after the pollination of the restorer line as the male parent, in order to ensure the purity of the hybrid, the male parent must be excised, resu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 杜德志赵志星晓蓉柳海东姚艳梅徐亮
Owner QINGHAI UNIVERSITY
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