Tissue culture method for rapid propagation and seedling of Yunnan polygonatum kingianum tubers and stem tips

Abstract

The invention discloses a tissue culture method for rapid propagation and seedling of Yunnan polygonatum kingianum tubers and stem tips. The tissue culture method comprises the following steps: 1) performing sterile germination of Yunnan polygonatum kingianum tubers and stem tips; 2) proliferation of Yunnan polygonatum kingianum protocorms; 3) performing differentiation of the Yunnan polygonatum kingianum protocorms; 4) strengthening seedlings of Yunnan polygonatum kingianum; 5) rooting large seedlings of Yunnan polygonatum kingianum. The tissue culture method has the advantages that by adjusting components of a seeding culture medium, Yunnan polygonatum kingianum tubers and stem tips seeded with the culture medium need no dark culture time but are subjected to direct light treatment, germinated seeds can form protocorms within about 14-25 days, and the germination rate is up to 96-98%; by adopting the tissue culture method, a very good promotion function is achieved for populations ofthe lily family medicinal plant in imminent danger, namely the Yunnan polygonatum kingianum, and a biological technique base is made for achievement of values of famous and precious traditional Chinese medicinal materials; bananas are adopted as a rooting culture medium preferably, the seedling time of the rooting culture medium with the bananas is 32-45 days, and the seedling time of a rooting culture medium with potatoes is 46-60 days.

Description

technical field [0001] The invention belongs to a method for propagating medicinal plant seedlings, and in particular relates to a tissue culture method for quickly propagating tubers and shoot tips of Rhizoma Polygonatum into seedlings. Background technique [0002] Polygonatum, also known as Jiejiegao, Immortal Rice, Liliaceae, Polygonatum, is the dry rhizome of herbaceous plants, mainly distributed in Yunnan, Guizhou, Sichuan and other places. The main ingredients are aspartic acid, homoserine, diaminobutyric acid, digitonin, etc., sweet and flat. It has the effects of invigorating the middle and replenishing qi, moistening the heart and lungs, and strengthening bones and muscles. Polygonatum yunnanensis has the following pharmacological effects: ① [antibacterial effect] Rhizoma Polygonatum has inhibitory effect on antacid bacteria in test tubes. Its decoction is administered to guinea pigs with experimental tuberculosis at the same time as it is infected with tuberculos...

AI Technical Summary

Problems solved by technology

[0003] ②[Anti-fungal effect] Polygonatum ethanol extract solution with a concentration of 2% or more begins to inhibit a variety of fungi, such as Trichophyton violae, Epidermophyton rubrum, etc., and its water extract is effective against Trichophyton gypsum and Koff Mann-Wolf's Epidermophyton has inhibitory effect, but it is reported that its 10% decoction has a slight inhibitory effect on Microsporum lanoides, but it is ineffective against other fungi

[0005] At present, there have been relevant reports on the tissue culture research of Rhizoma Polygonatum, but the high cost of tissue culture and the long cycle of seedling growth in test tubes are the bottlenecks restricting the large-scale industrial production of Polygonatum Rhizoma.