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Polyoxygenated cyclohexene derivatives and application thereof

A cyclohexene and oxygen substitution technology, applied in the field of microorganisms, can solve problems such as cyclohexene compounds that have not yet been seen

Active Publication Date: 2018-04-20
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the isolation of polyoxo-substituted cyclohexene compounds from Phomopsis fungus

Method used

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  • Polyoxygenated cyclohexene derivatives and application thereof
  • Polyoxygenated cyclohexene derivatives and application thereof
  • Polyoxygenated cyclohexene derivatives and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Separation and preparation of polyoxygen substituted cyclohexene compounds A-G:

[0025] 1. Slant culture of Phomopsis YE3250 strain: peel potatoes, cut into small pieces, add water to boil, filter with gauze to obtain potato filtrate, then add glucose and agar, constant volume with distilled water, and sterilize to obtain slant medium; The medium is made into a test tube slant, and the YE3250 strain is picked and inserted into the slant medium for cultivation to obtain the strain slant;

[0026] 2. Seed culture of Phomopsis YE3250 strain: Take a strain transplant block from the above slant strains and inoculate it into PDB medium (PDB medium: 200 g of peeled potatoes, 20 g of glucose, 15 g of agar, 1000 g of distilled water mL, natural pH, sterilized at 121°C for 30 minutes), cultured on a shaker at 28°C and 200 r / min for 4 days to obtain seed solution;

[0027] 3. Liquid fermentation culture of Phomopsis YE3250 strain: 200 mL of PDB medium was placed in a 500 mL E...

Embodiment 2

[0030] The inhibitory activity of the polyoxo-substituted cyclohexene compounds A-G of the present invention on a-glucosidase was detected in a 96-well plate by using a microplate reader. The samples to be tested were dissolved in phosphate buffer (PBS, pH 6.8) at three concentrations. Add 20 μL of 0.01M phosphate buffer and 20 μL of phosphate buffer of 2.5 mM substrate 4-nitrophenol-α-D glucopyranoside (PNPG) into the 96-well plate in advance, then add 10 μL of sample solutions of different concentrations, Incubate for 5 minutes at 37°C in a standard instrument. Then add 10 μL of 0.2 U / mL α-glucosidase solution prepared with 0.01M phosphate buffer (pH 6.8) to start the reaction, and react at 37°C for 15 minutes, then add 0.2M Na 2 CO 3 80 μL was used to stop the reaction, and the absorbance OD value was measured at 405 nm. Acarbose was used as the positive control, PBS was used instead of α-glucosidase in the blank group, and the negative control was 20 μL PBS+20 μL PNPG+...

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Abstract

The invention relates to polyoxygenated cyclohexene derivatives separated from a fermentation product of Phomopsis sp. YE3250, and an application thereof, and belongs to the technical field of microorganisms. Seven polyoxygenated cyclohexene derivatives are obtained through conventional fermentation, extraction and separation of the strain YE3250, and are respectively named as polyoxygenated cyclohexene A, polyoxygenated cyclohexene B, polyoxygenated cyclohexene C, polyoxygenated cyclohexene D, polyoxygenated cyclohexene E, polyoxygenated cyclohexene F and polyoxygenated cyclohexene G. The polyoxygenated cyclohexene derivatives have an obvious inhibitory activity on alpha-glucosidase, and have a potential use in the preparation of new drugs for preventing and treating diabetes.

Description

technical field [0001] The present invention relates to seven compounds, in particular to a class of polyoxo-substituted cyclohexene derivatives derived from endophytic Phomopsis sp. The invention belongs to the technical field of microorganisms. Background technique [0002] Plant endophytic fungi have the characteristics of diversity and are a large class of microbial resources yet to be developed. They can produce new active compounds with rich chemical structures and are important sources of biologically active secondary metabolites. In recent years, they have become domestic and foreign. One of the research hotspots, especially mining endophytic fungal resources from medicinal plants. Yunnan peony (Paeonia delavayiFranch.) is a traditional medicinal plant, and its root bark is one of the important raw materials of the traditional Chinese medicine Paeonia delavayi Franch. It has sedative, hypnotic and analgesic effects. We isolated an endophytic fungus from the root ba...

Claims

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Application Information

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IPC IPC(8): C07C35/18C07D307/87C07D303/14C07D493/04C12P7/22C12P17/02C12P17/04C12P17/18A61P3/10C12R1/645
CPCC07C35/18C07D303/14C07D307/87C07D493/04C12P7/22C12P17/02C12P17/04C12P17/181
Inventor 吴少华
Owner YUNNAN UNIV
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