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Glioma prognostic marker circ7:42148226|42148468 and application

A prognostic marker, glioma technology, applied in the direction of biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc.

Active Publication Date: 2018-04-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even so, the recurrence rate of patients is still high, the 2-year survival rate is only 5%, and the average survival period of malignant glioblastoma is only about 14 months

Method used

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  • Glioma prognostic marker circ7:42148226|42148468 and application
  • Glioma prognostic marker circ7:42148226|42148468 and application
  • Glioma prognostic marker circ7:42148226|42148468 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of reagents for detecting the expression of circ7:42148226|42148468 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0019] 1. RNA stabilization solution 50ml

[0020] 2. Isopropanol 100ml

[0021] 3. Chloroform 100ml

[0022] 4. Trizol (from Molecular Research Center company) 50ml

[0023] 5. Enzyme-free water 10ml

[0024] 6. 1μM random reverse transcription primer (Thermo Company) 50μl

[0025] 7. 5× reverse transcription buffer (Thermo company) 200ml

[0026] 8. 10mM base triphosphate deoxynucleotide (Thermo company) 100μl

[0027] 9. 40U / μl RNase inhibitor (Thermo Company) 500μl

[0028] 10. 200U / μl MMLV reverse transcriptase (Thermo Company) 50μl

[0029] 11. Premix Ex Taq (Thermo Company) 50μl

[0030] 12. 10μM circRNA circ7:42148226|42148468 real-time fluorescence quantitative PCR specific primer 30μl

[0031] circRNAcirc7:42148226|42148468 Forward primer:

[0032] 5'-CTCTGTGATAAGTCTGTCCAGG-3',

...

Embodiment 2

[0038] Example 2 The relationship between the expression level of circRNA circ7:42148226|42148468 in glioma tissue and prognosis

[0039] 1. Preservation of glioma tissue: collect the glioma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0040] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid was transferred to a tube tube, it was lysed on ice for 15 minutes. After the lysis, centrifuge at 12000rpm for 10min at 4°C, and transfer the supernatant to a new tube. Add 200 μl of chloroform to the tube, shake it by hand for 15-30 seconds, place it on ice for 15 minutes, centrifuge at 12000 rpm at 4°C for 15 minutes; carefully take the upper aqueous phase into a new tube, ad...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker circ7:42148226|42148468 and application. A reagent for detecting the expression quantity of circRNAcirc7:42148226|42148468 of a glioma source is used for preparing a prognostic preparation of a glioma patient. Research proves that the glioma patient with relatively low expression of circRNAcirc 7:42148226|42148468 has relatively high postoperative survival rate. A prognosis of the glioma patient is made by detecting the expression level of circRNAcirc7:42148226|42148468 in glioma tissue of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum circRNA marker for glioma prognosis, the application of a reagent for detecting the marker for preparing a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most common intracranial tumor with high incidence and high malignancy. The current treatment of glioma is mainly surgical resection, supplemented by radiotherapy and chemotherapy. However, even so, the recurrence rate of patients is still high, the 2-year survival rate is only 5%, and the average survival period of malignant glioblastoma is only about 14 months. One of the main factors that make glioma difficult to cure is the aggressive growth of glioma itself, and the boundary between tumor cells and normal brain tissue is very blurred and it is difficult to completely remove it by surgery; in addition, due to the rapid proliferation of glioma cells Tumor cells that survive in the hypo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 佘晓玲武明花付海娟李沛瑶王蓉刘庆范礼
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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