Liquid chromatographic analysis method for detecting escitalopram content in blood

Abstract

The invention provides a liquid chromatographic analysis method for detecting escitalopram content in blood. The method is characterized in that a standard solution is calibrated through a liquid chromatographic analysis device and a fluorescence detector, and a standard curve equation can be obtained as y=a*x+b by fitting; a blood sample to be detected is prepared and processed and then is detected to obtain y value of the blood to be detected through the liquid chromatographic analysis device and the fluorescence detector; the y value of the blood to be detected is substituted into the standard curve equation, and the relative content x of a target substance in the blood sample to be detected can be calculated; the content of working liquid of an internal standard substance is known, andon that basis, the concentration of escitalopram in the blood to be detected in the sample is calculated. With the adoption of the method, the accuracy of the quantitation result is improved; the system error is avoided; the analyzing time is extremely shortened; the detection process is simple, convenient and quick; experimental foundation is provided for personalized dosing of escitalopram andreducing toxic and side effects of escitalopram.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the concentration of antidepressant escitalopram in blood. Background technique [0002] The molecular formula of escitalopram is C 20 h 21 FN 2 O, the specific chemical structure is figure 1 As shown, its mechanism of action is to inhibit the reabsorption of 5-hydroxytryptamine and increase the concentration of extracellular 5-hydroxytryptamine in the brain, thereby resisting depression and improving mood. Its relative selectivity is the highest among similar drugs, and it has a strong antidepressant effect. Fewer reactions, better safety and tolerance. [0003] The currently commonly used methods for the determination of escitalopram blood concentration mainly include high performance liquid chromatography-fluorescence detection and liquid chromatography tandem mass spectrometry. The analysi...

AI Technical Summary

Problems solved by technology

The analysis cost of liquid chromatography tandem mass spectrometry is very high and has high requirements on the environment

High-performance liquid chromatography is accurate in quantification, good in reproducibility, and good in economic benefits. However, the existing methods still have problems such as complicated pretreatment and long analysis time, and are not suitable for large-throughput sample detection.

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