Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing paraffin slices of young crop roots

A technology for paraffin sectioning and tenderness, which is applied in the field of preparing paraffin sections of young and tender roots of crops to achieve the effect of saving time

Active Publication Date: 2021-08-17
SHANGHAI JIAOTONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few methods for rapid paraffin sectioning of young roots of crops.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] This embodiment relates to a method for preparing paraffin slices of young crop roots, which specifically includes the following steps:

[0042] S1. Material selection:

[0043] 1. Material selection

[0044] Depending on the purpose of the production. According to the purpose, take samples regularly according to the growth characteristics of the root system and the characteristics of the growth and development stages, such as the development of main roots, lateral roots, and observation of hair filament differentiation. It is necessary to take regular and continuous samples at different growth stages according to the growth characteristics of the roots; The materials (except for pathological sampling, generally require fresh, robust, and normal) can represent the structure of a certain type of root system; the sampling environment is consistent and typical.

[0045] 2. Material cutting

[0046] The young roots of crops have different cell shapes, arrangements, and s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for preparing paraffin slices of young crop roots, which comprises the following steps: material selection; microwave fixation; dyeing; microwave dehydration; microwave transparency; microwave wax dipping; embedding; slices and baked slices; dewaxing; sealing and drying; wherein, the step of microwave fixation specifically includes the following operations: the plant tissue is placed in a fixative solution, and the microwave under the condition of thawing is carried out in a water bath at 75°C. After 3 minutes of treatment, the fixative was replaced at least twice, and after each replacement, microwave treatment was carried out under the condition of thawing for 2 minutes in a water bath at 75°C. The invention has the following beneficial effects: the invention realizes the rapid production of paraffin sections. It takes about 6-7 days to make a complete section in the traditional paraffin section method, but the time required by the present invention is 3 days, which saves time on the basis of ensuring the effect of making slices.

Description

technical field [0001] The invention relates to a method for preparing paraffin slices of young roots of crops, and belongs to the technical field of paraffin slices of plant sample tissues. Background technique [0002] Paraffin section technology is one of the most important and commonly used methods in microscopic technology, which started in the 18th century and was cited by Klebs in 1869. This method is to embed the material in paraffin, and then slice it together with the paraffin on a microtome, so it is called paraffin section method. In the original paraffin section method, the section material was only surrounded by paraffin, not permeated by paraffin. It was not until Bourne published a report on paraffin embedding technology in 1882 that paraffin embedding was widely used, played an important role in the history of plant cell and tissue research, and will continue to be used as a routine technology in the future. effect. [0003] The traditional paraffin secti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 李彭丽于立尧姚琪张亚琪牛庆良
Owner SHANGHAI JIAOTONG UNIV