A kind of preparation method of anti-biological pollution coating on sensor electrode surface

Abstract

The invention discloses a preparation method of a sensor electrode surface anti-biological-pollution coating. The method is characterized by comprising two major steps of synthesizing photosensitive hyaluronic acid and preparing an anti-biological-pollution coating. The prepared photosensitive hyaluronic acid has high hydrophilcity, optical crosslinking performance and good biocompatibility. The synthesis of the photosensitive hyaluronic acid is simple; the raw materials can be easily obtained; the industrial production can be easily realized; through the introduction of photosensitive monomers, on the basis that the coating achieves the self excellent characteristics of the hyaluronic acid, the coating structure can be fixed through ultraviolet light crosslinking. When the photosensitivehyaluronic acid is applied to raw liquid sample detection, a sensor electrode based on modification by the photosensitive hyaluronic acid coating has long-term and efficient anti-biological-pollutioncapability. The light crosslinking technology, the functional coating technology and the biosensing technology are combined; the preparation method can be widely applied to the fields of food safety,biological medicine, environment protection monitoring, life safety and the like.

Description

technical field [0001] The invention relates to the fields of polymer materials, biological functional coatings and electrochemical sensors, in particular to a method for preparing an anti-biological pollution coating by modifying the surface of sensor electrodes with photosensitive hyaluronic acid. Background technique [0002] When traditional biosensors are directly in contact with raw actual samples (such as blood, milk, juice, and domestic sewage) to detect the components, the surface of the electrode will be polluted due to the adhesion of proteins, blood cells, and microorganisms, which will reduce the detection sensitivity and service life. , or even lead to complete failure. Take blood analysis as an example. At present, when analyzing actual blood, it is first necessary to use centrifugal separation technology to process the blood, and then use a biochemical analyzer to detect the target substance in the separated serum. There are some obvious defects in this proc...

AI Technical Summary

Problems solved by technology

There are some obvious defects in this processing method: the biochemical analysis process requires centrifugation facilities, which is very inconvenient for small clinics, families or online rapid testing; blood samples may be contaminated during centrifugation; due to the long time Therefore, it will cause detection errors caused by the metabolism of blood components that cannot be ignored. In addition, after the concentration of the target substance in the serum is detected, it needs to be converted by an empirical formula, but the final result cannot fully express the concentration of the whole blood. the real situation of

Although this method exhibits good anti-bioadhesion properties, this anti-adhesion is only effective in the short term. As the drug is consumed, the anti-protein contamination effect of this coating will gradually decrease.

In recent years, some zwitterionic polymers and hydrophilic polymers have been widely used in avidin adhesion coatings. However, these polymers lack suitable structures and are difficult to anchor on the surface of conductive substrates for a long time.

Images