Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition for reducing inhibition of nucleic acid amplification

A technology of nucleic acid amplification reaction and composition, applied in the direction of biochemical equipment and methods, microorganism measurement/inspection, etc.

Active Publication Date: 2018-05-01
3M INNOVATIVE PROPERTIES CO
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The time and sensitivity to analyze and suppress nucleic acid amplification caused by inhibitory substances in the sample have certain limitations to the usefulness of genetic testing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition for reducing inhibition of nucleic acid amplification
  • Composition for reducing inhibition of nucleic acid amplification
  • Composition for reducing inhibition of nucleic acid amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach AZ

[0162] Embodiments AZ are a kit comprising:

[0163] a plurality of zirconia particles;

[0164] nonionic surfactants; and

[0165] Organic iron chelating reagent;

[0166] Wherein the organic iron chelating reagent has a first affinity constant greater than or equal to 10 relative to ferric iron 4.2 and has a second affinity constant of less than 10 relative to magnesium 3.8 , where the first affinity constant and the second affinity constant were determined in deionized water at 20 °C at pH 8.45.

[0167] Embodiment BA is the kit of embodiments AZ, wherein the plurality of particles has an average particle size of less than about 1 μm.

[0168] Embodiment BB is the kit according to Embodiment AZ or Embodiment BA, wherein the organoiron chelating reagent comprises a plurality of carboxylate groups.

[0169]Embodiment BC is the kit according to any one of embodiments AZ to BB, wherein the organic iron chelating agent is selected from the group consisting of ethylene glyco...

Embodiment 1-8

[0197] Examples 1-8: Compositions comprising ferric ions and various amounts of zirconia particles .

[0198] Zirconia nanoparticle dispersion (10% w / w in water; ≤ 100 nm average particle size (BET); surface area 11 m²) was obtained from Sigma Chemical Co. 2 / mL; part number 643025). The test compositions were prepared by adding the components in the order specified in Table 1 to deionized water. Comparative Example 1 was prepared as described in Example 1 except that no zirconia particles and no potassium citrate were added to the composition. Each composition of Examples 1-8 and Comparative Example 1 contained 200 mg / L ferric ammonium citrate and 320 mg / L TRITON X-100. The amounts of potassium citrate and zirconia dispersions used in each Example and Comparative Example 1 are shown in Table 2.

[0199] Table 1 : The test composition of Examples 1-8 and Comparative Example 1. The order of addition of the components of the composition is also shown.

[0200]

[020...

Embodiment 9-14

[0211] Examples 9-14: Compositions comprising zirconia particles and various concentrations of surfactants and ferric ions .

[0212] Test compositions were prepared with the components shown in Table 4 and their respective order of addition to deionized water. In these examples, ferric ion and nonionic surfactant concentrations were varied according to Table 5. Zirconia surface area kept constant at 20m 2 / L. Samples (600 μL) were prepared as described in Example 1 and Comparative Examples 2-7. Aliquots (20 μL) of the lysozyme solution were added to 580 μL of the test composition in Neptune (2600.X) lysis tubes.

[0213] Table 4 : Test compositions of Examples 9-14 and Comparative Examples 2-7. The order of addition of the components of the composition is shown.

[0214]

[0215] table 5 : The amount of ferric ammonium citrate, TRITON X-100 and zirconia particle dispersion added to each composition of Examples 9-14 and Comparative Examples 2-7.

[0216]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Average particle sizeaaaaaaaaaa
Login to View More

Abstract

A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes a plurality of zirconium oxide particles, a non-ionic surfactant,and an organic iron-chelating reagent. The organic iron-chelating reagent has a first affinity constant greater than or equal to 104.2 with respect to ferric iron and a second affinity constant less than 103.8 with respect to magnesium, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20 DEG C. Optionally, the composition includes polyvinylpyrrolidone. Optionally, the composition comprises water. The composition has a pH of about 8.45 to 8.85. Methods of using the composition to prepare a sample for nucleic acid amplificationare also provided.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to U.S. Provisional Patent Application No. 62 / 159,733, filed May 11, 2015, and U.S. Provisional Patent Application No. 62 / 189,991, filed July 8, 2015, the disclosures of which are incorporated by reference in their entirety way incorporated into this article. [0003] This application has been associated with its Sequence Listing (file name Sequence_Listing_76042US003.TXT, created April 25, 2016). This Sequence Listing file contains 1,885 bytes and is hereby incorporated by reference in its entirety. Background technique [0004] Conventional methods for detecting pathogens and other microorganisms are culture-based methods, but these methods are time-consuming, laborious, and no longer meet the needs of quality control and diagnostic laboratories to provide rapid results. [0005] Efforts to overcome the problems of culturing microorganisms, false positives in pathogen detectio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6806C12Q1/6844
CPCC12Q1/6806C12Q1/6844C12Q2527/125C12Q2531/119
Inventor 格雷戈里·W·西顿夏文胜托尼亚·D·博尼利亚
Owner 3M INNOVATIVE PROPERTIES CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com