Composition containing stem cell components and application thereof

A stem cell and composition technology, applied in the field of compositions containing stem cell components, can solve the problem of not preventing human beings from pursuing beauty rights, etc., and achieve good effects, anti-oxidation and anti-aging effects, and widely used effects.

Inactive Publication Date: 2018-05-04
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Aging research has become a major research topic in the field of life sciences. Even

Method used

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  • Composition containing stem cell components and application thereof
  • Composition containing stem cell components and application thereof
  • Composition containing stem cell components and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] Preparation of stem cell concentrate: collect the cell culture supernatant before passage to obtain stem cell culture; filter to remove cell debris and microorganisms; place the filtrate in a 5K ultrafiltration tube at low temperature (-4°C to 8°C) and high speed Centrifuge at a speed of 800-5000 r / min to obtain a stem cell concentrate, which is stored at -20°C for future use. The stem cells in this experimental example are embryonic stem cells.

[0019] Activity detection method: H 2 o 2 Induced Cellular Senescence Model

[0020] 1.H 2 o 2 Establishment of induced cell senescence model, established H 2 o 2 Stimulation time and concentration

[0021] Facic acid dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to insoluble blue-purple formazan, and the amount of blue-purple formazan formed is proportional to the number of living cells and the biosynthetic metabolic capacity of cells, while dead cells No such function. Therefore, the sur...

Embodiment 1

[0031] A composition containing stem cell components, based on a total mass fraction of 100%, comprising 20% ​​to 100% of a stem cell concentrate, 0 to 80% of a basal medium, and 1 μmol / mL of vitamin E and 1.0×10 -2 μg / mL of ginseng extract. In this embodiment, the concentrated solution of stem cells is the concentrated solution of embryonic stem cells. Embryonic stem cells were obtained from the Cell Bank of the Chinese Academy of Sciences.

[0032] Several groups of compositions were designed by using the SAS method, which are:

[0033] (1) Stem cell concentrate with a mass fraction of 20% and high-sugar DMEM complete medium with a mass fraction of 80%, also added with 1 μmol / mL vitamin E and 10 -2 μg / mL ginseng extract;

[0034] (2) Stem cell concentrate with a mass fraction of 40% and high-sugar DMEM complete medium with a mass fraction of 60%, also added with 1 μmol / mL vitamin E and 1.0×10 -2 μg / mL ginseng extract;

[0035] (3) Stem cell concentrate with a mass fract...

Embodiment 2

[0043] A composition containing stem cell components, based on a total mass fraction of 100%, comprising 30% of stem cell concentrate and 70% of high-sugar DMEM complete medium, and vitamin E with a concentration of 10 μmol / mL and a concentration of 1.0×10 -5 ~10 -1 μg / mL of ginseng extract. In this embodiment, the stem cell concentrate is the adult stem cell concentrate. Adult stem cells were obtained from the Guangdong Provincial Cord Blood Hematopoietic Stem Cell Bank.

[0044] Several groups of compositions were designed by using the SAS method, which are:

[0045] (1) The concentration of stem cells with a mass fraction of 30% and the complete high-sugar DMEM medium with a mass fraction of 70% were also added with vitamin E at a concentration of 10 μmol / mL and a concentration of 1.0×10 -5 Ginseng extract in μg / mL;

[0046] (2) The concentration of stem cells with a mass fraction of 30% and the complete high-sugar DMEM medium with a mass fraction of 70% were also added...

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Abstract

The invention discloses a composition containing stem cell components and application thereof. Based a total mass fraction of 100%, the composition comprises 20-100% of a stem cell concentrated solution and 0-80% of a complete medium, and vitamin E and ginseng extract are added into the composition. Oxidation resistance of the product is determined by an anti-oxidation experiment, an oxidative stress cell aging model is established, and the stem cell composition is subjected to anti-aging function study, so that the stem cell composition with multiple effects, small toxic and side effects andan anti-aging effect is found.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a composition containing stem cell components and its application. Background technique: [0002] Aging research has become a major research topic in the field of life sciences. Even though aging is inevitable, it cannot stop human beings from pursuing the right to beauty. In recent years, the research on anti-aging drugs has been the focus of people's attention. Anti-aging drugs are an important tool for delaying aging and aiming at improving life efficiency. Studies have shown that antioxidants can scavenge free radicals, prevent free radicals from destroying biofilms, and have a certain anti-aging effect. Antioxidant experimental tests are carried out on selected drugs. Studies have shown that stem cells, natural products and many compounds have anti-aging effects, and skin aging is a direct response to the aging of the body. Therefore, human skin fibroblast HSF is ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/67A61K8/9789A61Q19/08A23L33/105A23L33/15
CPCA23L33/105A23L33/15A23V2002/00A61K8/678A61K8/97A61K8/99A61Q19/08A23V2250/2124A23V2250/712A23V2200/302A23V2200/30
Inventor 李志远程娜王飞肖杰邓思浩孙曼曼苏柳惠林赛月叶威卢大华
Owner CENT SOUTH UNIV
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