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Method for evaluating skin conditions by skin keratinocyte form

A technology of keratinocytes and chamfering, which is applied in material analysis by optical means, preparation of test samples, measurement devices, etc., and can solve problems that affect test results, easily generate bubbles, and appear unclear

Pending Publication Date: 2018-05-04
ZHUHAI EASYCARE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese invention patent application CN2013100128496 discloses a cell encapsulation method and cell observation method, by encapsulating cells between the adhesive layer of scotch tape and the adhesive layer of scotch tape, and then using a phase contrast microscope to observe the enclosed cells Cells, no staining solution is used; however, only the general shape of the cells can be observed by this method, especially for the nuclei and the appearance of melanin are not clear
Moreover, with this packaging method, air bubbles are likely to be generated during the operation, which will affect the test results.

Method used

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  • Method for evaluating skin conditions by skin keratinocyte form
  • Method for evaluating skin conditions by skin keratinocyte form
  • Method for evaluating skin conditions by skin keratinocyte form

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] see figure 1 , a keratinocyte sampling detection tool, comprising a loading plate 1, a scotch tape 2 and a small hole 3, wherein the loading plate is provided with a collection hole 4 with a radius of 9 mm, and one side of the collection hole 4 is provided with a height of 1mm chamfer 5, the adhesive layer of the scotch tape is fixed on the non-chamfered side of the collection hole, and the scotch tape completely covers the collection hole. The loading board 1 is a PC board with a length of 76.2mm, a width of 25.48mm and a thickness of 2mm, and the scotch tape is a test film for exfoliation of skin horny layer cells from CK Company of Germany. The diameter of the small hole is 1.35 mm, and the center of the collection hole is at the same height on the loading plate.

Embodiment 2

[0093] see figure 2 , a keratinocyte sampling detection tool, comprising a loading plate 1 and scotch tape 2, wherein the loading plate is provided with a collection hole 4 (square) with a length of 9 mm on one side, and one side of the collection hole 4 is provided with a height of 1mm chamfer 5, the adhesive layer of the scotch tape is fixed on the non-chamfered side of the collection hole, and the scotch tape completely covers the collection hole. The loading board 1 is a PC board with a length of 76.2mm, a width of 25.48mm and a thickness of 2mm, and the scotch tape is a test film for exfoliation of skin horny layer cells from CK Company of Germany.

Embodiment 3

[0095] Volunteer A, female, 30 years old, uses the keratinocyte sampling detection tool of the present invention to evaluate the skin condition, including the following steps:

[0096](1) Sampling: Press the chamfered side of the keratinocyte sampling detection tool in Example 1 on the skin, align the small hole with the intersection of the corner of the eye and the wing of the nose, and stroke it from top to bottom with the fingertips Press the collection hole three times; hold down around the corner of the eye with your hand, and slowly tear off the tool from bottom to top at the same time;

[0097] (2) Ferrous sulfate dyeing: the ferrous sulfate solution with a concentration of 0.01g / ml is dropped into the collection hole from the side with the chamfer, and heated by microwave at a temperature of 50°C. After 6 minutes of dyeing time, use Wash with distilled water, wash three times, three minutes each time;

[0098] (3) Counterstaining with Nuclear Fast Red: Drop Nuclear Fa...

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Abstract

The invention provides a method for evaluating skin conditions by skin keratinocyte form. The method comprises the following steps of after a special skin keratinocyte patch is sampled, heating the patch by microwaves, dyeing by ferrous sulfate, redyeing by HE (hematoxylin-eosin), and observing the skin keratinocyte form under a microscope, including the polymerizing degree, arrangement type, area, health degree and melanin of the skin keratinocyte, so as to evaluate the skin conditions. The method has the advantages that the sampling is convenient, the observed picture is clear after dyeing,the operation can be completed by only using one patch, the efficiency is improved, and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of skin detection, and in particular relates to a method for staining keratinocytes, a method for evaluating skin conditions through the morphology of keratinocytes, and a keratinocyte sampling and detection tool. Background technique [0002] With the development of the economy, consumers are paying more and more attention to skin health. How to accurately evaluate the skin condition and choose suitable skin care products is particularly important for consumers and cosmetics manufacturers. [0003] At present, the main methods of skin testing at home and abroad are as follows: 1. Collect skin image data through an external image acquisition device and transmit it to a computer for analysis. Professional and stable image acquisition equipment is large in size and inconvenient to carry. The mobile terminal (smartphone) is convenient for taking pictures, but the stability and repeatability of light and photo q...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N21/84
CPCG01N1/30G01N21/84
Inventor 何海鸥马自竹王德友周立武
Owner ZHUHAI EASYCARE TECH CO LTD
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