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Application of placenta multipotential stem cell preparation in preparation of medicine for treatment of acute lung injury

A technology for pluripotent stem cells and acute lung injury, which can be used in drug combinations, medical raw materials derived from mammals, and respiratory diseases.

Inactive Publication Date: 2018-05-15
HANGZHOU S EVANS BIOSCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of studies have attempted to elucidate the pathogenesis of ALI, no particularly effective treatment has been found so far.

Method used

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  • Application of placenta multipotential stem cell preparation in preparation of medicine for treatment of acute lung injury
  • Application of placenta multipotential stem cell preparation in preparation of medicine for treatment of acute lung injury
  • Application of placenta multipotential stem cell preparation in preparation of medicine for treatment of acute lung injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation of acute lung injury mouse model

[0017] The mice were anesthetized by injecting 250 μl of 1% pentobarbital sodium (prepared with PBS), the skin and muscle layers were cut along the middle of the neck, the trachea was exposed, and a 1 ml syringe was used to puncture the middle of the two rings of the trachea in the centripetal direction, inject 2.5 mg / ml of LPS (prepared in PBS) at a dose of 10 mg / kg body weight. Immediately after the injection, the mice were rotated upright to make the solution evenly distributed on both sides of the lungs. After suture, the mice were fed normally in a sterile environment.

Embodiment 2

[0018] Example 2: Placental pluripotent stem cell preparation

[0019] (1) Isolation, culture and flow cytometric identification of placental pluripotent stem cells

[0020] Isolation and culture of placental pluripotent stem cells: wash the placental surface with physiological saline several times to remove impurities such as congestion and mucus, then quickly disinfect the placental surface with a volume concentration of 75% ethanol solution, and then wash off the residual ethanol solution with physiological saline; From the completed placenta, take the fetal surface tissue into a clean centrifuge tube, and cut it into minced pieces; add 2 times the volume of enzyme solution (type I collagenase with a concentration of 300 IU / mL + DNase I with a concentration of 40 IU / mL, prepared with normal saline) ), digested at 37 °C for 1 hour; the digested product was filtered with a 70 μm filter to remove tissue residues, and a cell suspension was obtained; The isolated mononuclear ce...

Embodiment 3

[0026] Example 3: Placental pluripotent stem cell preparation infusion reduces the degree of lung injury and improves lung function

[0027] (1) A mouse acute lung injury (ALI) model established by the method described in Example 1, the experiment was divided into 3 groups: group 1 was the normal group, group 2 was the ALI model group, and group 3 was the ALI model placental pluripotent stem cell preparation injection group. 3 groups 6 hours after modeling, press 8×10 7 The placental pluripotent stem cell preparation prepared in Example 2 was infused through the tail vein at a dose per kg body weight. 90h after injection, samples were taken for lung histopathology and blood gas analysis to analyze the effect of placental pluripotent stem cells on ALI.

[0028] (2) Pathological detection of lung tissue: Take lung tissue, fix it in 4% paraformaldehyde at 4 degrees overnight, then perform paraffin section and HE staining, observe and photograph under microscope, and detect pulm...

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PUM

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Abstract

The invention discloses an application of a placenta multipotential stem cell preparation in preparation of a medicine for treatment of acute lung injury. The placenta multipotential stem cell is sourced from the fetal surface part of a newborn placenta tissue and is a fibroblast-like cell of adherent growth. The placenta multipotential stem cell can express CD73, CD90 and CD105 and not express CD14, CD19, CD34, CD45 and HLA-DR. In the invention, the placenta multipotential stem cell is convenient to obtain and has abundant sources. The placenta multipotential stem cell preparation is simple in production, is simple in infusion technology and easy to use, can significantly reduce degree of acute lung injury and improve functions of lung, and has important clinical significance and extensive application prospect.

Description

(1) Technical field [0001] The invention relates to the field of acute lung injury treatment, in particular to the use of placental pluripotent stem cells in the treatment of acute lung injury and a preparation method thereof. (2) Background technology [0002] Acute lung injury (ALI) is mainly caused by gastric acid inhalation, infection, trauma, fat embolism, inhalation of toxic gases, etc. Infection is the most common cause. Regardless of the cause of ALI, the inflammatory response plays a major role in its pathological changes. Although a large number of studies have attempted to elucidate the pathogenesis of ALI, no particularly effective treatment has been found so far. At present, the treatment of ALI is mainly based on supportive treatment, drug treatment and assisted ventilation treatment. Clinically, the fatality rate is still very high, about 30% to 40%. Therefore, it is very important to develop effective drugs and methods for ALI treatment. urgent. [0003] I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61P11/00
CPCA61K35/50
Inventor 潘若浪张强杜小春戴玲华王金福
Owner HANGZHOU S EVANS BIOSCI LTD
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