Method for extracting tanshinone IIA from radix salviae miltiorrhizae

A technology of tanshinone and salvia miltiorrhiza, applied in the production of steroids, bulk chemicals, organic chemistry, etc., can solve the problems of low extraction purity and low extraction rate, and achieve the effects of good thermal stability, fast adsorption, and large attachment capacity

Inactive Publication Date: 2018-05-15
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for extracting tanshinone IIA from Danshen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0018] Implementation Case 1: A method using supercritical CO 2 The new method of fluid extraction, ultrafiltration separation, macroporous resin adsorption and column chromatography purification of tanshinone IIA is as follows:

[0019] (1) Cut the whole herb of Salvia miltiorrhiza to 3cm, add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 1%, the extraction pressure is 20MPa, the temperature is 50°C, the extraction time is 100min, CO 2 The flow rate is 1mg / g crude drug min to obtain the extract;

[0020] (2) Adjust the pH value of the tanshinone IIA extract treated in step (1) to 3.0 with hydrochloric acid, let stand, suction filter, and concentrate under reduced pressure to obtain the crude extract of tanshinone IIA;

[0021] (3) Ultrafiltration membrane separation is adopted, the operating pressure of the membrane is 0.2MPa, the molecular weight cut-off is 1000, and the r...

Embodiment example 2

[0027] Implementation Case 2: A method using supercritical CO 2 The new method of fluid extraction, ultrafiltration separation, macroporous resin adsorption and column chromatography purification of tanshinone IIA is as follows:

[0028] (1) Cut Salvia miltiorrhiza to 2cm, add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the volume percentage of the entrainer in the total extraction solvent is 2%, the extraction pressure is 30MPa, the temperature is 50°C, the extraction time is 120min, CO 2 The flow rate is 1mg / g crude drug min to obtain the extract;

[0029] (2) adjusting the pH value of the gentiopicroside extract treated in step (1) to 4.0 with hydrochloric acid, leaving it to stand, suction filtration, and concentrating under reduced pressure to obtain a crude gentiopicroside extract;

[0030] (3) Ultrafiltration membrane separation is adopted, the operating pressure of the membrane is 0.2MPa, the molecular weight cut-off is 3000, and the rej...

Embodiment example 3

[0036] Implementation Case 3: A method using supercritical CO 2 The new method of fluid extraction, ultrafiltration separation, macroporous resin adsorption and column chromatography purification of tanshinone IIA is as follows:

[0037] (1) Cut Salvia miltiorrhiza to 3cm, add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, the entrainer accounts for 3% by volume of the total extraction solvent, the extraction pressure is 40MPa, the temperature is 60°C, the extraction time is 120min, CO 2 The flow rate is 1mg / g crude drug min to obtain the extract;

[0038] (2) Adjust the pH value of the tanshinone IIA extract treated in step (1) to 4.0 with hydrochloric acid, leave it to stand, suction filter, and concentrate under reduced pressure to obtain the crude extract of tanshinone IIA;

[0039] (3) Adopt ultrafiltration membrane to separate, membrane operating pressure is 0.2MPa, molecular weight cut off 5000, rejection rate is 95%;

[0040] (4) Tanshinon...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction, relates to a method for purifying tanshinone IIA, and particularly relates to a method for extracting tanshinone IIA from radix salviae miltiorrhizae. By applying supercritical CO2 fluid extraction, original color and flavor can not be damaged by heating, heat sensitivity can be better ensured, and substanceswhich are easy to oxidize can not be damaged; and extracts can also be simultaneously separated and purified in the extraction process. The method provided by the invention has the advantages that macroporous adsorption resin which is stable in physicochemical property, larger in surface area, higher in exchange speed, high in mechanical strength, high in anti-pollution capability and good in heatstability is selected, and the macroporous adsorption resin is insoluble in acids, alkalis and organic media, has better selectively to organic substances, is free from influence of inorganic salts and strong-ion low-molecular compounds, can selectively adsorb tanshinone IIA from the solution through physical absorption, and has the effects of high absorption speed, high desorption speed and larger adsorption capacity.

Description

Technical field: [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a method for purifying tanshinone IIA, in particular to a method for extracting tanshinone IIA from salvia miltiorrhiza. Background technique: [0002] Salvia miltiorrhiza (scientific name: Salvia miltiorrhiza Bunge): a dicotyledonous perennial erect herb of the genus Salvia in the family Lamiaceae, with thick roots, vermilion outside, white inside, fleshy, and leaves that are often odd-numbered pinnate compound leaves, terminal or axillary in total Inflorescence; bracts lanceolate, calyx bell-shaped, purplish, corolla purple-blue, style far outstretched, nutlets black, oval, flowering from April to August, and fruit after flowering. [0003] Distributed in Anhui, Shanxi, Hebei, Sichuan, Jiangsu and other places in China. In addition, Hubei, Gansu, Liaoning, Shaanxi, Shandong, Zhejiang, Henan, Jiangxi and other places. Japan is also distributed. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
CPCC07J73/003Y02P20/54
Inventor 姚德坤许丽李玉伟苏学辉万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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