SNP marker tightly linked to cucumber phytophthora capsici resistance character and application of SNP marker

Abstract

The invention discloses a SNP marker tightly linked to the cucumber phytophthora capsici resistance character and application of the SNP marker. A molecular marker Cs3419019 screened by invention is tightly linked to the cucumber phytophthora capsici resistance character and can be directly used for cucumber phytophthora capsici resistance assistant breeding work. A CAPs marker amplification primer designed according to the marker can be simply, conveniently and rapidly used for screening a cucumber phytophthora capsici resistance material. The SNP marker provided by the invention can accelerate the breeding process, lowers the breeding cost, improves the breeding efficiency and can also propel transformation from traditional breeding to molecule breeding. The technical method of the invention has the advantages of rapidness, accuracy, simpleness in operation and the like and has a relatively large application prospect.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a SNP molecular marker closely linked with the anti-blight traits of cucumber and its application. Background technique [0002] Blight is one of the important diseases of cucumber, which is caused by Phytophthoramelonis Katsura, and occurs in all cucumber producing areas in the country, especially in southern China, where there is a lot of rain and humidity in spring and summer, which is easy to cause the outbreak and spread of the disease . Once the disease occurs, it will be difficult to control with chemical agents, and the loss will be serious. In general, the production will be reduced by 30%, and the harvest will be almost complete in the epidemic year, which poses a serious threat to cucumber production and breeding. At present, the lack of anti-epidemic materials in cucumber resources has brought great difficulties to the work of cucumber anti-epide...

AI Technical Summary

Problems solved by technology

Once the disease occurs, it will be difficult to control with chemical agents, and the loss will be serious. In general, the yield will be reduced by 30%, and the harvest will be almost eliminated in the epidemic year, which poses a serious threat to cucumber production and breeding.

At present, the lack of anti-epidemic materials in cucumber resources has brought great difficulties to the work of cucumber anti-epidemic breeding

[0003] Traditional disease resistance breeding generally adopts the method of artificial inoculation to identify the resistance traits of cucumber, but as a breeding or transgenic material, if it is transplanted to the field after inoculation, the pathogenic bacteria will quickly spread to other materials, and it will be difficult to prevent and control later. and production work caused great losses

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