In-vitro oriented screening method of peanut high-oil body

A technology for directional screening and peanuts, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult cultivation of high-yield and high-oil peanut varieties, lack of high-oil germplasm resources of peanuts, etc., and save domestication and cultivation time , Reduce the time of hardening seedlings, save resources and cost

Pending Publication Date: 2018-05-29
QINGDAO AGRI UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art, and provide a method for in vitro directional screening of peanut high-oil

Method used

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  • In-vitro oriented screening method of peanut high-oil body
  • In-vitro oriented screening method of peanut high-oil body
  • In-vitro oriented screening method of peanut high-oil body

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Embodiment 1

[0024] A method for in vitro directional screening of peanut high oil bodies according to the present invention comprises the following steps:

[0025] 1. Preparation of somatic embryo induction medium

[0026] (1) Prepare a somatic embryo induction medium, select MS medium as the basic medium, and add 2,4-D to the MS medium to form a somatic embryo induction medium. The amount of 2,4-D added is 2-12 mg / L. The somatic embryo induction medium in this example is MS+5mg / L2,4-D+30g / L sucrose+8g / L agar; the pH of the somatic embryo induction medium was adjusted to 5.8.

[0027] 2. Preparation of medium for somatic embryo germination into seedlings and high oil body directional selection

[0028] (1) Preparation of somatic embryo germination and high-oil screening medium: MS medium was selected as the basic medium, and hydroxyproline and 6-benzylaminopurine (BAP) were added to this MS medium to form somatic embryo germination and high-oil screening medium. Oriented screening medi...

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Abstract

The invention relates to an in-vitro oriented screening method of a peanut high-oil body, which is capable of solving the problems that peanuts are lack of high-oil germplasm resources and high-yieldand high-oil peanut varieties are difficult to cultivate through conventional breeding. The high-oil body is screened through an in-vitro oriented screening way, a screening process is proceeded in aculture medium, a large amount of culture materials without resistance are eliminated, a large amount of resources and cost can be saved and reduced, mutagenic culture and oriented screening can be carried out all the year round, and the operation is convenient. New peanut high-oil germplasms can be created and screened, genetic basis of peanuts can be broadened, and the difficulty that breeding of the high-yield and high-oil new varieties is difficult to break through as the peanuts are lack of the high-oil germplasm resources can be overcome.

Description

technical field [0001] The invention belongs to the field of peanut breeding, in particular to a method for in vitro directional screening of peanut high oil bodies. Background technique [0002] Peanut is an important oil crop in my country and occupies an important position in the national economy. More than 50% of the peanuts produced in my country are used for oil extraction. Generally, the oil content of more than 50% is a high-oil variety or high-oil material. Every 1 percentage point increase in the oil content can increase the net profit by 7%. Therefore, new varieties of high-oil peanuts are cultivated. It has become one of the main breeding goals of the majority of breeders. However, there is a lack of high-oil variety resources in peanuts, and it is difficult to obtain high-oil offspring by cross-breeding. Therefore, the breeding of high-yield and high-oil varieties has always been a difficult problem to solve. The use of mutagenesis technology can cause gene mut...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06A01H3/00A01G2/30A01G22/40
CPCA01H1/04A01H4/001A01H4/008
Inventor 禹山林王晶珊隋炯明乔利仙杨庆利姜德锋赵明霞衣艳君张芳汤松王晓杰
Owner QINGDAO AGRI UNIV
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